Abstract
Objective
To determine whether a cholinergic mechanism interferes with the pulmonary response to 5-hydroxytryptamme (5-HT) in the bovine species.
Design
The protocols differed with regard to the type of pretreatment calves were given 10 minutes before administration of 5-HT (0.05 mg/kg of body weight/min, over 2 minutes). Pretreatment consisted of saline, atropine, or hexamethonium solution given IV.
Animals
6 healthy unsedated Friesian calves.
Procedure
Pulmonary function values were obtained before, during, and after 5-HT infusions.
Results
After saline pretreatment, response to 5-HT consisted of immediate and brief apnea, bradycardia, and hypotension, followed by sustained tachypnea, tachycardia, pulmonary hypertension, and hypocapnic hypoxemia. Lung dynamic compliance (CLdyn) decreased to 19% of its baseline value, and total pulmonary resistance (Rl) increased to 235%. Hexamethonium pretreatment resulted in a similar pattern of response except for the immediate and brief 5-HT-induced triad of apnea, bradycardia, and hypotension, which was suppressed. After atropine pretreatment, immediate and brief 5-HT-induced apnea-bradycardia-hypotension triad and sustained hypoxemia were abolished. In contrast, sustained tachypnea, tachycardia, pulmonary hypertension, and hypocapnia were maintained. Changes of CLdyn (59%) and Rl (138%) were significantly attenuated.
Conclusions
The initial and short-lasting response to 5-HT (ie, the apnea-bradycardia-hypotension triad) is mediated through a reflex central cholinergic pathway. The 5-HT-induced changes in Cldyn and Rl are consistent with development of diffuse bronchoconstriction. Attenuation of these changes by atropine suggests that this bronchoconstrictor response to 5-HT is partly mediated through a cholinergic postganglionic pathway. (Am J Vet Res 1996;57:896–901)