Abstract
Objective
To evaluate the effects of dilution and alkalinization, separately and together, on the stability of uric acid in canine urine stored at −20 C.
Design
Prospective-controlled study.
Animals
5 dogs with confirmed ammonium urate uroliths, 6 Beagles, and 6 mixed-breed dogs.
Procedure
Dogs were fed a 31.4% protein (dry weight), meat-based diet for 21 days, and urine samples were collected on day 22. Urine samples were preserved, using combinations of dilution and alkalinization, and divided into 1-ml aliquots for storage at −20 C for 1 to 12 weeks. Urine uric acid concentrations were measured, using high-performance liquid chromatography, on day of collection (baseline), and after 1, 2, 4, 8, and 12 weeks.
Results
Alkalinization did not have a significant effect on reproducibility of measurements of uric acid concentrations in urine; however, dilution did have a significant effect. Compared with baseline, uric acid concentrations in urine samples collected from dogs with ammonium urate uroliths and Beagles and diluted 1:10 or 1:20 with deionized water were not different after storage for 1 to 12 weeks. Uric acid concentrations in urine samples collected from mixed-breed dogs did not differ from baseline values during the 12-week storage period whether samples were undiluted or were diluted 1:10 or 1:20 with deionized water.
Conclusions
Measurements of uric acid concentration are most reproducible in canine urine samples stored at −20 C for 1 to 12 weeks when samples are diluted 1:20 with deionized water.
Clinical Relevance
To ensure reproducibility of measurements of uric acid concentration in urine samples collected from dogs affected with urate uroliths, urine should be diluted 1:20 with deionized water. Alkalinization is not necessary, and is not recommended because of the additional step in processing and its potential to interfere with measurement of other urinary analytes. (Am J Vet Res 1996;57:787–790)
