Serum neutralizing antibody response and protection against experimentally induced liver abscesses in steers vaccinated with Fusobacterium necrophorum

S. Saginala From the Departments of Animal Sciences (Saginala, Nagaraja), Diagnostic Medicine/Pathology and Microbiology (Tan, Chengappa), and Statistics (Kemp), Kansas State University, Manhattan, KS 66506; Midwest Veterinary Services Inc, Oakland, NE 68045 (Lechtenberg); and Mallinckrodt Veterinary Inc. Mundelein, IL 60060 (Hine).

Search for other papers by S. Saginala in
Current site
Google Scholar
PubMed
Close
 MVSc
,
T. G. Nagaraja From the Departments of Animal Sciences (Saginala, Nagaraja), Diagnostic Medicine/Pathology and Microbiology (Tan, Chengappa), and Statistics (Kemp), Kansas State University, Manhattan, KS 66506; Midwest Veterinary Services Inc, Oakland, NE 68045 (Lechtenberg); and Mallinckrodt Veterinary Inc. Mundelein, IL 60060 (Hine).

Search for other papers by T. G. Nagaraja in
Current site
Google Scholar
PubMed
Close
 MVSc, PhD
,
Z. L. Tan From the Departments of Animal Sciences (Saginala, Nagaraja), Diagnostic Medicine/Pathology and Microbiology (Tan, Chengappa), and Statistics (Kemp), Kansas State University, Manhattan, KS 66506; Midwest Veterinary Services Inc, Oakland, NE 68045 (Lechtenberg); and Mallinckrodt Veterinary Inc. Mundelein, IL 60060 (Hine).

Search for other papers by Z. L. Tan in
Current site
Google Scholar
PubMed
Close
 BVSc, PhD
,
K. F. Lechtenberg From the Departments of Animal Sciences (Saginala, Nagaraja), Diagnostic Medicine/Pathology and Microbiology (Tan, Chengappa), and Statistics (Kemp), Kansas State University, Manhattan, KS 66506; Midwest Veterinary Services Inc, Oakland, NE 68045 (Lechtenberg); and Mallinckrodt Veterinary Inc. Mundelein, IL 60060 (Hine).

Search for other papers by K. F. Lechtenberg in
Current site
Google Scholar
PubMed
Close
 DVM, PhD
,
M. M. Chengappa From the Departments of Animal Sciences (Saginala, Nagaraja), Diagnostic Medicine/Pathology and Microbiology (Tan, Chengappa), and Statistics (Kemp), Kansas State University, Manhattan, KS 66506; Midwest Veterinary Services Inc, Oakland, NE 68045 (Lechtenberg); and Mallinckrodt Veterinary Inc. Mundelein, IL 60060 (Hine).

Search for other papers by M. M. Chengappa in
Current site
Google Scholar
PubMed
Close
 DVM, PhD
,
K. E. Kemp From the Departments of Animal Sciences (Saginala, Nagaraja), Diagnostic Medicine/Pathology and Microbiology (Tan, Chengappa), and Statistics (Kemp), Kansas State University, Manhattan, KS 66506; Midwest Veterinary Services Inc, Oakland, NE 68045 (Lechtenberg); and Mallinckrodt Veterinary Inc. Mundelein, IL 60060 (Hine).

Search for other papers by K. E. Kemp in
Current site
Google Scholar
PubMed
Close
 PhD
, and
P. M. Hine From the Departments of Animal Sciences (Saginala, Nagaraja), Diagnostic Medicine/Pathology and Microbiology (Tan, Chengappa), and Statistics (Kemp), Kansas State University, Manhattan, KS 66506; Midwest Veterinary Services Inc, Oakland, NE 68045 (Lechtenberg); and Mallinckrodt Veterinary Inc. Mundelein, IL 60060 (Hine).

Search for other papers by P. M. Hine in
Current site
Google Scholar
PubMed
Close
 PhD

Abstract

Objective

To determine the efficacy of leukotoxin-based Fusobacterium necrophorum vaccines and dietary tylosin in providing protection against experimentally induced hepatic abscesses in steers.

Design

30 steers assigned randomly to 6 treatment groups of 5 steers each: 1, phosphate-buffered saline solution (PBSS; control); 2, PBSS control, fed tylosin (100 mg/steer) daily; 3, inactivated whole-cell culture with oil emulsion adjuvant; 4, culture supernatant (crude toxoid) with oil emulsion adjuvant; 5, semipurified leukotoxoid with oil emulsion adjuvant; and 6, semipurified leukotoxoid with saponin adjuvant.

Procedure

Steers were inoculated SC with emulsified antigen or PBSS on days 0 and 21. Blood samples were collected at weekly intervals to monitor serum antileukotoxin antibody titer. On day 42, all steers were challenge exposed intraportally with F necrophorum culture. Three weeks later (day 63), steers were euthanatized and necropsied to examine liver and assess protection.

Results

Antileukotoxin antibody titers of all vaccinated groups markedly increased from baseline values, and mean titers of vaccinated groups were higher than those of the control and tylosin-treated groups. Steers vaccinated with culture supernatant with oil emulsion adjuvant or semipurified leukotoxoid with saponin adjuvant had the highest mean antibody titers. All 5 steers in the control group developed liver abscesses. Tylosin feeding did not protect steers challenge exposed with F necrophorum intraportally.

Conclusions

Culture supernatant was more protective than whole-cell culture or semipurified leukotoxin against experimentally induced hepatic abscesses. Partial purification of leukotoxin appeared to reduce its protective immunity. (Am J Vet Res 1996;57:483–488)

Abstract

Objective

To determine the efficacy of leukotoxin-based Fusobacterium necrophorum vaccines and dietary tylosin in providing protection against experimentally induced hepatic abscesses in steers.

Design

30 steers assigned randomly to 6 treatment groups of 5 steers each: 1, phosphate-buffered saline solution (PBSS; control); 2, PBSS control, fed tylosin (100 mg/steer) daily; 3, inactivated whole-cell culture with oil emulsion adjuvant; 4, culture supernatant (crude toxoid) with oil emulsion adjuvant; 5, semipurified leukotoxoid with oil emulsion adjuvant; and 6, semipurified leukotoxoid with saponin adjuvant.

Procedure

Steers were inoculated SC with emulsified antigen or PBSS on days 0 and 21. Blood samples were collected at weekly intervals to monitor serum antileukotoxin antibody titer. On day 42, all steers were challenge exposed intraportally with F necrophorum culture. Three weeks later (day 63), steers were euthanatized and necropsied to examine liver and assess protection.

Results

Antileukotoxin antibody titers of all vaccinated groups markedly increased from baseline values, and mean titers of vaccinated groups were higher than those of the control and tylosin-treated groups. Steers vaccinated with culture supernatant with oil emulsion adjuvant or semipurified leukotoxoid with saponin adjuvant had the highest mean antibody titers. All 5 steers in the control group developed liver abscesses. Tylosin feeding did not protect steers challenge exposed with F necrophorum intraportally.

Conclusions

Culture supernatant was more protective than whole-cell culture or semipurified leukotoxin against experimentally induced hepatic abscesses. Partial purification of leukotoxin appeared to reduce its protective immunity. (Am J Vet Res 1996;57:483–488)

Advertisement