Abstract
Objective
To develop a model to study the kinetics and relative amounts of cytokines produced by liver cells during enteric infection.
Design
Salmonella enteriditis lipopolysaccharide (LPS)-or live S choleraesuis-stimulated isolated livers from clinically normal pigs and pigs with active acute phase response.
Animals
7- to 14-day-old salmonellosis-free pigs, 4 to 12/group.
Procedure
Livers were removed and perfused with oxygenated Krebs-Henseleit solution for 30 minutes and with S choleraesuis or LPS added for 7 minutes. Livers were then perfused with 500 ml of fresh solution in a closed loop procedure for 180 minutes. Perfusate samples were collected for tumor necrosis factor-α (TNFα) and interleukin 6 (IL-6) bioassays.
Results
Tumor necrosis factor-α values remained constant during perfusion of normal livers and increased in those exposed to LPS. Interleukin 6 values increased in perfusate from normal livers from 30 to 150 minutes, then decreased. In livers from pigs with an active acute phase response, TNFα values were reduced; IL-6 appeared by 2 minutes and decreased after 25 minutes.
Conclusions
Isolated livers could be kept viable for 3 hours, and IL-6 and TNFα could be measured by the bioassays used.
Clinical Relevance
Model can be used for studying and modifying the response of liver cells to infective agents. (Am J Vet Res 1996;57:472–476)
