Editorial Type:
Physiology

Modulation of matrix metalloprotease 13 (collagenase 3) gene expression in equine chondrocytes by interleukin 1 and corticosteroids

John P. Caron From the Department of Large Animal Clinical Sciences, College of Veterinary Medicine, Michigan State University, East Lansing, MI 48824 (Caron), and Osteoarthritis Research Unit, Department of Medicine, Notre Dame Hospital, University of Montreal, Quebec, Canada H2L 4K8 (Tardif, Martel-Pelletier, DiBattista, Geng, Pelletier).

Search for other papers by John P. Caron in
Current site
Google Scholar
PubMed Close
 DVM, MVSc
,
Ginette Tardif From the Department of Large Animal Clinical Sciences, College of Veterinary Medicine, Michigan State University, East Lansing, MI 48824 (Caron), and Osteoarthritis Research Unit, Department of Medicine, Notre Dame Hospital, University of Montreal, Quebec, Canada H2L 4K8 (Tardif, Martel-Pelletier, DiBattista, Geng, Pelletier).

Search for other papers by Ginette Tardif in
Current site
Google Scholar
PubMed Close
 PhD
,
Johanne Martel-Pelletier From the Department of Large Animal Clinical Sciences, College of Veterinary Medicine, Michigan State University, East Lansing, MI 48824 (Caron), and Osteoarthritis Research Unit, Department of Medicine, Notre Dame Hospital, University of Montreal, Quebec, Canada H2L 4K8 (Tardif, Martel-Pelletier, DiBattista, Geng, Pelletier).

Search for other papers by Johanne Martel-Pelletier in
Current site
Google Scholar
PubMed Close
 PhD
,
John A. DiBattista From the Department of Large Animal Clinical Sciences, College of Veterinary Medicine, Michigan State University, East Lansing, MI 48824 (Caron), and Osteoarthritis Research Unit, Department of Medicine, Notre Dame Hospital, University of Montreal, Quebec, Canada H2L 4K8 (Tardif, Martel-Pelletier, DiBattista, Geng, Pelletier).

Search for other papers by John A. DiBattista in
Current site
Google Scholar
PubMed Close
 PhD
,
Changshan Geng From the Department of Large Animal Clinical Sciences, College of Veterinary Medicine, Michigan State University, East Lansing, MI 48824 (Caron), and Osteoarthritis Research Unit, Department of Medicine, Notre Dame Hospital, University of Montreal, Quebec, Canada H2L 4K8 (Tardif, Martel-Pelletier, DiBattista, Geng, Pelletier).

Search for other papers by Changshan Geng in
Current site
Google Scholar
PubMed Close
 MD, MSc
, and
Jean-Pierre Pelletier From the Department of Large Animal Clinical Sciences, College of Veterinary Medicine, Michigan State University, East Lansing, MI 48824 (Caron), and Osteoarthritis Research Unit, Department of Medicine, Notre Dame Hospital, University of Montreal, Quebec, Canada H2L 4K8 (Tardif, Martel-Pelletier, DiBattista, Geng, Pelletier).

Search for other papers by Jean-Pierre Pelletier in
Current site
Google Scholar
PubMed Close
 MD
Free access

Abstract

Objective

To determine whether matrix metalloprotease 13 (MMP-13; collagenase 3) is produced by equine chondrocytes and to investigate modulation of its expression by recombinant human interleukin β (rhIL-1β) and corticosteroids.

Procedure

Equine chondrocytes in monolayer culture were stimulated with rhIL-1β. Total RNA was extracted, purified, and reverse transcribed into DNA. Using appropriate primers, a putative MMP-13 fragment was amplified by polymerase chain reaction, and cloned into a bacterial vector. The resultant fragment was purified and sequenced, then was used to prepare a digoxigenin-labeled cRNA probe. Monolayer cultures of first-passage chondrocytes were treated with rhIL-1β in the presence or absence of dexamethasone (10-6M) or methylprednisolone acetate (10-9M to 10-5M), in addition to positive and negative controls. Cellular RNA was extracted and resolved on agarose gels and subjected to northern blot analysis, using the equine MMP-13 probe.

Results

Reverse transcriptase-polymerase chain reaction enabled isolation of a 0.6-kb fragment of equine MMP-13 cDNA that had 93% homology with the human MMP-13 cDNA sequence. rhIL-1 significantly stimulated MMP-13 expression in the chondrocytes. Methylprednisolone acetate inhibited the stimulatory effects of rhIL-1 in dose-dependent manner that was statistically significant at 10-5M.

Conclusions

Novel information was gained on the existence of MMP-13 and its expression in equine chondrocytes, which suggests a possible role for this enzyme in matrix degradation in horses with arthritis. (Am J Vet Res 1996;57:1631–1634)

All Time Past Year Past 30 Days
Abstract Views 0 0 0
Full Text Views 3202 3104 46
PDF Downloads 138 119 0
Cover American Journal of Veterinary Research
American Journal of Veterinary Research
Issue:
01 Nov 1996
Advertisement