Evaluation of recombinant bluetongue virus antigens, using dot immunobinding assay with serum from sheep and cattle

Alpana Naresh From the Department of Veterinary Microbiology, CCS Haryana Agricultural University, Hisar, Haryana 125 004, India (Naresh, Prasad); Laboratory of Molecular Biophysics, University of Oxford, and NERC Institute of Virology and Environmental Microbiology, Mansfield Rd, Oxford, OXI 3SR, United Kingdom (Roy); and University of Alabama, Birmingham, AL 35294 (Roy).

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 MSc
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Polly Roy From the Department of Veterinary Microbiology, CCS Haryana Agricultural University, Hisar, Haryana 125 004, India (Naresh, Prasad); Laboratory of Molecular Biophysics, University of Oxford, and NERC Institute of Virology and Environmental Microbiology, Mansfield Rd, Oxford, OXI 3SR, United Kingdom (Roy); and University of Alabama, Birmingham, AL 35294 (Roy).

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 PhD
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Gaya Prasad From the Department of Veterinary Microbiology, CCS Haryana Agricultural University, Hisar, Haryana 125 004, India (Naresh, Prasad); Laboratory of Molecular Biophysics, University of Oxford, and NERC Institute of Virology and Environmental Microbiology, Mansfield Rd, Oxford, OXI 3SR, United Kingdom (Roy); and University of Alabama, Birmingham, AL 35294 (Roy).

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 BVSc, PhD

Abstract

Objective

To evaluate 2 genetically engineered group-specific antigens: baculovirus- and yeast-expressed VP7 and the conventionally produced group-specific antigen of bluetongue virus (BTV), using dot immunobinding assay (DIA).

Sample Population and Procedure

A total of 260 serum samples of sheep and cattle from various livestock farms in different Indian states (eg, Haryana, Himachal Pradesh, Jammu and Kashmir, Punjab, and Rajasthan) were tested for the presence of BTV antibodies by DIA.

Results

Of 260 sera tested, 92 (35%) were positive for BTV antibodies using the baculovirus-expressed antigen, 96 (37%) were positive using the yeast-expressed antigen, and 103 (40%) were positive by use of conventionally produced antigen.

Conclusion and Clinical Relevance

The overall agreement for all the 3 antigens was 92%, indicating that the recombinant group-specific proteins can be used for serodiagnosis of BTV, using DIA. (Am J Vet Res 1996;57:1556–1558)

Abstract

Objective

To evaluate 2 genetically engineered group-specific antigens: baculovirus- and yeast-expressed VP7 and the conventionally produced group-specific antigen of bluetongue virus (BTV), using dot immunobinding assay (DIA).

Sample Population and Procedure

A total of 260 serum samples of sheep and cattle from various livestock farms in different Indian states (eg, Haryana, Himachal Pradesh, Jammu and Kashmir, Punjab, and Rajasthan) were tested for the presence of BTV antibodies by DIA.

Results

Of 260 sera tested, 92 (35%) were positive for BTV antibodies using the baculovirus-expressed antigen, 96 (37%) were positive using the yeast-expressed antigen, and 103 (40%) were positive by use of conventionally produced antigen.

Conclusion and Clinical Relevance

The overall agreement for all the 3 antigens was 92%, indicating that the recombinant group-specific proteins can be used for serodiagnosis of BTV, using DIA. (Am J Vet Res 1996;57:1556–1558)

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