Editorial Type:
Physiology

Effects of stromelysin activity on proteoglycan degradation of canine articular cartilage explants

R. J. Todhunter From the Department of Clinical Sciences (Todhunter) and the James A. Baker Institute for Animal Health (Grisanzio, Burton-Wurster, Lust), College of Veterinary Medicine, Cornell University, Ithaca, NY 14853, and Osteoarthritis Sciences Incorporated, Cambridge, MA 02129 (Yeh, Sheldon, Walker).

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 BVSc, PhD
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L.-A. Yeh From the Department of Clinical Sciences (Todhunter) and the James A. Baker Institute for Animal Health (Grisanzio, Burton-Wurster, Lust), College of Veterinary Medicine, Cornell University, Ithaca, NY 14853, and Osteoarthritis Sciences Incorporated, Cambridge, MA 02129 (Yeh, Sheldon, Walker).

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A. Sheldon From the Department of Clinical Sciences (Todhunter) and the James A. Baker Institute for Animal Health (Grisanzio, Burton-Wurster, Lust), College of Veterinary Medicine, Cornell University, Ithaca, NY 14853, and Osteoarthritis Sciences Incorporated, Cambridge, MA 02129 (Yeh, Sheldon, Walker).

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L. Grisanzio From the Department of Clinical Sciences (Todhunter) and the James A. Baker Institute for Animal Health (Grisanzio, Burton-Wurster, Lust), College of Veterinary Medicine, Cornell University, Ithaca, NY 14853, and Osteoarthritis Sciences Incorporated, Cambridge, MA 02129 (Yeh, Sheldon, Walker).

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S. L. Walker From the Department of Clinical Sciences (Todhunter) and the James A. Baker Institute for Animal Health (Grisanzio, Burton-Wurster, Lust), College of Veterinary Medicine, Cornell University, Ithaca, NY 14853, and Osteoarthritis Sciences Incorporated, Cambridge, MA 02129 (Yeh, Sheldon, Walker).

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N. Burton-Wurster From the Department of Clinical Sciences (Todhunter) and the James A. Baker Institute for Animal Health (Grisanzio, Burton-Wurster, Lust), College of Veterinary Medicine, Cornell University, Ithaca, NY 14853, and Osteoarthritis Sciences Incorporated, Cambridge, MA 02129 (Yeh, Sheldon, Walker).

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G. Lust From the Department of Clinical Sciences (Todhunter) and the James A. Baker Institute for Animal Health (Grisanzio, Burton-Wurster, Lust), College of Veterinary Medicine, Cornell University, Ithaca, NY 14853, and Osteoarthritis Sciences Incorporated, Cambridge, MA 02129 (Yeh, Sheldon, Walker).

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SUMMARY

We investigated whether stromelysin activity in the medium of canine articular cartilage explants is associated with proteoglycan degradation in these explants. Cartilage explants were treated with recombinant human interleukin 1α (rh-il-lα), lipopolysaccharide, or canine monocyte-conditioned medium. Proteoglycan synthesis and degradation were measured. Metalloproteinase activity (inhibitable by tissue inhibitor of metalloproteinase 2) in the culture medium was measured by use of fluorimetry with a quenched fluorescent substrate. Western blots of the medium were probed with polyclonal antibodies to human stromelysin, collagenase, and gelatinase.

Neither metalloproteinase activity nor proteoglycan degradation were inducible in canine cartilage explants treated with rh-ll-1α. However, proteoglycan synthesis was significantly (P < 0.05) decreased by concentrations of 10 and 100 ng of rh-il-1α/ml. Metalloproteinase activity in the medium accompanied proteoglycan degradation of cartilage treated with lipopolysaccharide and monocyte-conditioned medium. The metalloproteinase released into the medium was identified as prostromelysin by results of western blotting.

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Cover American Journal of Veterinary Research
American Journal of Veterinary Research
Issue:
01 Sep 1995
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