Persistence of a single electropherotype and serotype (G6P5) of bovine rotavirus in calves on a closed dairy farm from 1990 to 1993

Hiroshi Ishizaki From the Department of Veterinary Microbiology, Obihiro University of Agriculture and Veterinary Medicine, Obihiro 080 (Ishizaki, Ohta, Shirahata, Goto), and the Department of Hygiene, School of Medicine, Sapporo Medical University, Sapporo 060 (Taniguchi, T Urasawa, S Urasawa), Japan.

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Chikako Ohta From the Department of Veterinary Microbiology, Obihiro University of Agriculture and Veterinary Medicine, Obihiro 080 (Ishizaki, Ohta, Shirahata, Goto), and the Department of Hygiene, School of Medicine, Sapporo Medical University, Sapporo 060 (Taniguchi, T Urasawa, S Urasawa), Japan.

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Toshikazu Shirahata From the Department of Veterinary Microbiology, Obihiro University of Agriculture and Veterinary Medicine, Obihiro 080 (Ishizaki, Ohta, Shirahata, Goto), and the Department of Hygiene, School of Medicine, Sapporo Medical University, Sapporo 060 (Taniguchi, T Urasawa, S Urasawa), Japan.

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Hitoshi Goto From the Department of Veterinary Microbiology, Obihiro University of Agriculture and Veterinary Medicine, Obihiro 080 (Ishizaki, Ohta, Shirahata, Goto), and the Department of Hygiene, School of Medicine, Sapporo Medical University, Sapporo 060 (Taniguchi, T Urasawa, S Urasawa), Japan.

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Koki Taniguchi From the Department of Veterinary Microbiology, Obihiro University of Agriculture and Veterinary Medicine, Obihiro 080 (Ishizaki, Ohta, Shirahata, Goto), and the Department of Hygiene, School of Medicine, Sapporo Medical University, Sapporo 060 (Taniguchi, T Urasawa, S Urasawa), Japan.

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Tomoko Urasawa From the Department of Veterinary Microbiology, Obihiro University of Agriculture and Veterinary Medicine, Obihiro 080 (Ishizaki, Ohta, Shirahata, Goto), and the Department of Hygiene, School of Medicine, Sapporo Medical University, Sapporo 060 (Taniguchi, T Urasawa, S Urasawa), Japan.

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Shozo Urasawa From the Department of Veterinary Microbiology, Obihiro University of Agriculture and Veterinary Medicine, Obihiro 080 (Ishizaki, Ohta, Shirahata, Goto), and the Department of Hygiene, School of Medicine, Sapporo Medical University, Sapporo 060 (Taniguchi, T Urasawa, S Urasawa), Japan.

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SUMMARY

A virologic survey was conducted on calves with diarrhea associated with bovine rotavirus (brv) on a closed dairy farm. The brv was detected from 32 of 219 (14.6%) fecal specimens repeatedly collected from 56 calves born during the years 1992-1993, regardless of whether they had diarrhea. Most of the 32 strains were isolated from fecal specimens obtained from 2- to 6-week-old calves. After electrophoresis of double-stranded viral rna from the 32 strains, genomic rna migration patterns were similar to those of the predominant brv strains isolated at the same farm during the years 1990-1991. All representative strains were identified as G serotype 6 (G6) and P type 5 (P5) by results of the virus-neutralization test and polymerase chain reaction procedure. Thus, brv had no change in genomic rna electropherotypes and serologic antigenicities in a closed dairy herd over a period of several years.

SUMMARY

A virologic survey was conducted on calves with diarrhea associated with bovine rotavirus (brv) on a closed dairy farm. The brv was detected from 32 of 219 (14.6%) fecal specimens repeatedly collected from 56 calves born during the years 1992-1993, regardless of whether they had diarrhea. Most of the 32 strains were isolated from fecal specimens obtained from 2- to 6-week-old calves. After electrophoresis of double-stranded viral rna from the 32 strains, genomic rna migration patterns were similar to those of the predominant brv strains isolated at the same farm during the years 1990-1991. All representative strains were identified as G serotype 6 (G6) and P type 5 (P5) by results of the virus-neutralization test and polymerase chain reaction procedure. Thus, brv had no change in genomic rna electropherotypes and serologic antigenicities in a closed dairy herd over a period of several years.

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