Vaccination of cattle with outer membrane protein-enriched fractions of Pasteurella haemolytica and resistance against experimental challenge exposure

Rebecca J. Morton From the Departments of Veterinary Parasitology, Microbiology, and Public Health (Morton, Fulton, Ewing, Homer) and Pathology (Panciera, Confer), College of Vetennary Medicine, Oklahoma State University, Stillwater, OK 74078, and the USDA, Agricultural Research Service, National Animal Disease Center, Central Plains Area, PO Box 70, Ames, IA 50010 (Frank).

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Roger J. Panciera From the Departments of Veterinary Parasitology, Microbiology, and Public Health (Morton, Fulton, Ewing, Homer) and Pathology (Panciera, Confer), College of Vetennary Medicine, Oklahoma State University, Stillwater, OK 74078, and the USDA, Agricultural Research Service, National Animal Disease Center, Central Plains Area, PO Box 70, Ames, IA 50010 (Frank).

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Robert W. Fulton From the Departments of Veterinary Parasitology, Microbiology, and Public Health (Morton, Fulton, Ewing, Homer) and Pathology (Panciera, Confer), College of Vetennary Medicine, Oklahoma State University, Stillwater, OK 74078, and the USDA, Agricultural Research Service, National Animal Disease Center, Central Plains Area, PO Box 70, Ames, IA 50010 (Frank).

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Glynn H. Frank From the Departments of Veterinary Parasitology, Microbiology, and Public Health (Morton, Fulton, Ewing, Homer) and Pathology (Panciera, Confer), College of Vetennary Medicine, Oklahoma State University, Stillwater, OK 74078, and the USDA, Agricultural Research Service, National Animal Disease Center, Central Plains Area, PO Box 70, Ames, IA 50010 (Frank).

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Sidney A. Ewing From the Departments of Veterinary Parasitology, Microbiology, and Public Health (Morton, Fulton, Ewing, Homer) and Pathology (Panciera, Confer), College of Vetennary Medicine, Oklahoma State University, Stillwater, OK 74078, and the USDA, Agricultural Research Service, National Animal Disease Center, Central Plains Area, PO Box 70, Ames, IA 50010 (Frank).

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John T. Homer From the Departments of Veterinary Parasitology, Microbiology, and Public Health (Morton, Fulton, Ewing, Homer) and Pathology (Panciera, Confer), College of Vetennary Medicine, Oklahoma State University, Stillwater, OK 74078, and the USDA, Agricultural Research Service, National Animal Disease Center, Central Plains Area, PO Box 70, Ames, IA 50010 (Frank).

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Anthony W. Confer From the Departments of Veterinary Parasitology, Microbiology, and Public Health (Morton, Fulton, Ewing, Homer) and Pathology (Panciera, Confer), College of Vetennary Medicine, Oklahoma State University, Stillwater, OK 74078, and the USDA, Agricultural Research Service, National Animal Disease Center, Central Plains Area, PO Box 70, Ames, IA 50010 (Frank).

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SUMMARY

Administration of an N-lauroylsarcosine-derived outer membrane protein fraction of Pasteurella haemolytica A1 (sci-1) induced a protective response in calves against intrathoracic challenge exposure with the homologous serovar. Outer membrane proteins from heterologous serovars, A6 and A9, induced partial protection that was associated with their respective similarities to serovar A1 in outer membrane protein profiles derived by use of sodium dodecyl sulfate-polyacrylamide gel electrophoresis. Calves vaccinated with sci preparations did not have detectable neutralizing antibody to P haemolytica A1 leukotoxin. Antibodies to whole-cell antigens, carbohydrate-protein subunit antigen, and sci-1 were associated with resistance, which indicates that protein antigens shared among cell surface, carbohydrate-protein subunit, and sci preparations are immunogenic and enhance resistance to experimental challenge exposure.

SUMMARY

Administration of an N-lauroylsarcosine-derived outer membrane protein fraction of Pasteurella haemolytica A1 (sci-1) induced a protective response in calves against intrathoracic challenge exposure with the homologous serovar. Outer membrane proteins from heterologous serovars, A6 and A9, induced partial protection that was associated with their respective similarities to serovar A1 in outer membrane protein profiles derived by use of sodium dodecyl sulfate-polyacrylamide gel electrophoresis. Calves vaccinated with sci preparations did not have detectable neutralizing antibody to P haemolytica A1 leukotoxin. Antibodies to whole-cell antigens, carbohydrate-protein subunit antigen, and sci-1 were associated with resistance, which indicates that protein antigens shared among cell surface, carbohydrate-protein subunit, and sci preparations are immunogenic and enhance resistance to experimental challenge exposure.

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