Detection of Toxoplasma gondii-specific IgA in the serum of cats

Derek P. Burney From the Department of Clinical Sciences, College of Veterinary Mediane and Biomedical Sciences, Colorado State University, Fort Collins, CO 80523.

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Michael R. Lappin From the Department of Clinical Sciences, College of Veterinary Mediane and Biomedical Sciences, Colorado State University, Fort Collins, CO 80523.

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Christi Cooper From the Department of Clinical Sciences, College of Veterinary Mediane and Biomedical Sciences, Colorado State University, Fort Collins, CO 80523.

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Melissa M. Spilker From the Department of Clinical Sciences, College of Veterinary Mediane and Biomedical Sciences, Colorado State University, Fort Collins, CO 80523.

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SUMMARY

An elisa for detection of Toxoplasma gondii-specific IgA in feline serum was developed. A group of cats (n = 7) was inoculated orally with T gondii bradyzoites. Toxoplasma gondii-specific serum IgM, IgG, and IgA responses were followed sequentially by use of the ELISA for 34 weeks. Serum IgA was detected later than IgM or IgG, and was detected in most cats on week 34 after inoculation. None of the cats was seropositive for IgA during the oocyst-shedding period. A group of client-owned cats with suspected clinical toxoplasmosis and a group of healthy cats were tested for Toxoplasma gondii-specific IgA in serum. A trend toward association of T gondii-specific IgA in serum of cats with ocular disease was observed.

SUMMARY

An elisa for detection of Toxoplasma gondii-specific IgA in feline serum was developed. A group of cats (n = 7) was inoculated orally with T gondii bradyzoites. Toxoplasma gondii-specific serum IgM, IgG, and IgA responses were followed sequentially by use of the ELISA for 34 weeks. Serum IgA was detected later than IgM or IgG, and was detected in most cats on week 34 after inoculation. None of the cats was seropositive for IgA during the oocyst-shedding period. A group of client-owned cats with suspected clinical toxoplasmosis and a group of healthy cats were tested for Toxoplasma gondii-specific IgA in serum. A trend toward association of T gondii-specific IgA in serum of cats with ocular disease was observed.

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