Detection of bovine immunodeficiency virus in blood and milk-derived leukocytes by use of polymerase chain reaction

Jerry W. Nash From the Department of Biological Sciences (Nash St. Cyr Coats), and the College of Veterinary Medicine (Hanson), Mississippi State University, Mississippi State, MS 39762-5759.

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Larry A. Hanson From the Department of Biological Sciences (Nash St. Cyr Coats), and the College of Veterinary Medicine (Hanson), Mississippi State University, Mississippi State, MS 39762-5759.

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Karen St. Cyr Coats From the Department of Biological Sciences (Nash St. Cyr Coats), and the College of Veterinary Medicine (Hanson), Mississippi State University, Mississippi State, MS 39762-5759.

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SUMMARY

Bovine immunodeficiency virus (biv) is prevalent in beef and dairy cattle, yet the mode(s) of biv transmission are undefined. Using polymerase chain reaction, which specifically targeted a 235-bp, highly conserved region of the biv pol gene, biv-infected leukocytes were detected in the blood and milk of biv- seropositive cows. These data confirm the presence of biv in milk and identify the potential for lactogenic transmission of this vims.

SUMMARY

Bovine immunodeficiency virus (biv) is prevalent in beef and dairy cattle, yet the mode(s) of biv transmission are undefined. Using polymerase chain reaction, which specifically targeted a 235-bp, highly conserved region of the biv pol gene, biv-infected leukocytes were detected in the blood and milk of biv- seropositive cows. These data confirm the presence of biv in milk and identify the potential for lactogenic transmission of this vims.

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