Comparison of argyrophilic nucleolar organizer regions by counting and image analysis in canine mammary tumors

Eric Destexhe From Service d’Anatomie Pathologique, Faculté de Médecine Véténnaire, Université de Liège, Boulevard du Rectorat, B 43, Sart-Tilman, B 4000, Liège, Belgium.

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Pascal Vanmanshoven From Service d’Anatomie Pathologique, Faculté de Médecine Véténnaire, Université de Liège, Boulevard du Rectorat, B 43, Sart-Tilman, B 4000, Liège, Belgium.

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Freddy Coignoul From Service d’Anatomie Pathologique, Faculté de Médecine Véténnaire, Université de Liège, Boulevard du Rectorat, B 43, Sart-Tilman, B 4000, Liège, Belgium.

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SUMMARY

Two techniques for evaluating argyrophilic nucleolar organizer regions (AgNOR) were compared on 74 canine mammary tumors to discriminate between benign and malignant lesions. For each lesion, direct counting of AgNOR on at least 100 cell nuclei was compared with area, perimeter, and integrated optical density AgNOR dot values determined by image analysis. Significant differences between benign and malignant tumors were observed with both methods; however, lesions determined as aggressive or proliferative by histologic evaluation were only singled out by image analysis measurements. Image analysis, in our hands, was a reliable, precise, and convenient technique to characterize malignancy in canine mammary tumors.

SUMMARY

Two techniques for evaluating argyrophilic nucleolar organizer regions (AgNOR) were compared on 74 canine mammary tumors to discriminate between benign and malignant lesions. For each lesion, direct counting of AgNOR on at least 100 cell nuclei was compared with area, perimeter, and integrated optical density AgNOR dot values determined by image analysis. Significant differences between benign and malignant tumors were observed with both methods; however, lesions determined as aggressive or proliferative by histologic evaluation were only singled out by image analysis measurements. Image analysis, in our hands, was a reliable, precise, and convenient technique to characterize malignancy in canine mammary tumors.

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