Characterization of functions of neutrophils from bone marrow of cattle with leukocyte adhesion deficiency

Hajime Nagahata From the Department of Veterinary Medicine, Rakuno Gakuen University, Bunkyodai-Midori 582, Ebetsu, Hokkaido 069 (Nagahata, Yamashita, Noda); Department of Microbiology, Faculty of Pharmaceutical Sciences, Health Sciences University of Hokkaido 061-02 (Nochi, Tamoto), Japan; and Department of Veterinary Pathobiology, College of Veterinary Medicine, The Ohio State University, 1925 Coffey Rd, Columbus, OH 43210-1093 (Kociba).

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Hiromi Nochi From the Department of Veterinary Medicine, Rakuno Gakuen University, Bunkyodai-Midori 582, Ebetsu, Hokkaido 069 (Nagahata, Yamashita, Noda); Department of Microbiology, Faculty of Pharmaceutical Sciences, Health Sciences University of Hokkaido 061-02 (Nochi, Tamoto), Japan; and Department of Veterinary Pathobiology, College of Veterinary Medicine, The Ohio State University, 1925 Coffey Rd, Columbus, OH 43210-1093 (Kociba).

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Koichi Tamoto From the Department of Veterinary Medicine, Rakuno Gakuen University, Bunkyodai-Midori 582, Ebetsu, Hokkaido 069 (Nagahata, Yamashita, Noda); Department of Microbiology, Faculty of Pharmaceutical Sciences, Health Sciences University of Hokkaido 061-02 (Nochi, Tamoto), Japan; and Department of Veterinary Pathobiology, College of Veterinary Medicine, The Ohio State University, 1925 Coffey Rd, Columbus, OH 43210-1093 (Kociba).

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Kazuto Yamashita From the Department of Veterinary Medicine, Rakuno Gakuen University, Bunkyodai-Midori 582, Ebetsu, Hokkaido 069 (Nagahata, Yamashita, Noda); Department of Microbiology, Faculty of Pharmaceutical Sciences, Health Sciences University of Hokkaido 061-02 (Nochi, Tamoto), Japan; and Department of Veterinary Pathobiology, College of Veterinary Medicine, The Ohio State University, 1925 Coffey Rd, Columbus, OH 43210-1093 (Kociba).

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Hiroshi Noda From the Department of Veterinary Medicine, Rakuno Gakuen University, Bunkyodai-Midori 582, Ebetsu, Hokkaido 069 (Nagahata, Yamashita, Noda); Department of Microbiology, Faculty of Pharmaceutical Sciences, Health Sciences University of Hokkaido 061-02 (Nochi, Tamoto), Japan; and Department of Veterinary Pathobiology, College of Veterinary Medicine, The Ohio State University, 1925 Coffey Rd, Columbus, OH 43210-1093 (Kociba).

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Gary J. Kociba From the Department of Veterinary Medicine, Rakuno Gakuen University, Bunkyodai-Midori 582, Ebetsu, Hokkaido 069 (Nagahata, Yamashita, Noda); Department of Microbiology, Faculty of Pharmaceutical Sciences, Health Sciences University of Hokkaido 061-02 (Nochi, Tamoto), Japan; and Department of Veterinary Pathobiology, College of Veterinary Medicine, The Ohio State University, 1925 Coffey Rd, Columbus, OH 43210-1093 (Kociba).

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SUMMARY

Marked differences in bone marrow cellularity were observed between cattle affected with leukocyte adhesion deficiency (lad) and control cattle. The number of nucleated cells in bone marrow was 2.9 to 8.8 times higher in cattle affected with lad, compared with controls. The myeloid-to-erythroid ratio of bone marrow from 3 cattle affected with lad ranged from 2.4 to 12. Deficient CD18 expression on neutrophils isolated from bone marrow of cattle with lad was clearly detected by flow cytometric analysis. Neutrophils from bone marrow of cattle affected with lad appeared round and not flat, after adherence to plastic wells under agarose, whereas neutrophils from bone marrow of clinically normal cattle were firmly spread on the surface of plastic wells. In the chemotaxis under-agarose assay, many pseudopodia were detected on bone marrow neutrophils from clinically normal cattle, but were not detected on bone marrow neutrophils from cattle with lad. Activities of chemotactic movements and phagocytosis of neutrophils isolated from bone marrow of cattle affected with lad were documented to be severely impaired.

SUMMARY

Marked differences in bone marrow cellularity were observed between cattle affected with leukocyte adhesion deficiency (lad) and control cattle. The number of nucleated cells in bone marrow was 2.9 to 8.8 times higher in cattle affected with lad, compared with controls. The myeloid-to-erythroid ratio of bone marrow from 3 cattle affected with lad ranged from 2.4 to 12. Deficient CD18 expression on neutrophils isolated from bone marrow of cattle with lad was clearly detected by flow cytometric analysis. Neutrophils from bone marrow of cattle affected with lad appeared round and not flat, after adherence to plastic wells under agarose, whereas neutrophils from bone marrow of clinically normal cattle were firmly spread on the surface of plastic wells. In the chemotaxis under-agarose assay, many pseudopodia were detected on bone marrow neutrophils from clinically normal cattle, but were not detected on bone marrow neutrophils from cattle with lad. Activities of chemotactic movements and phagocytosis of neutrophils isolated from bone marrow of cattle affected with lad were documented to be severely impaired.

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