Determination of carbonic anhydrase III isoenzyme concentration in sera of racehorses with exertional rhabdomyolysis

Toshiho Nishita From the Laboratories of Veterinary Physiology 1 (Nishita) and Veterinary Anatomy 1 (Asari), School of Veterinary Medicine, Azabu University, 1-17-71, Fuchinobe, Sagamihara, Kanagawa 229, and Ohohashi Veterinary Hospital, 4-49, Yakusiji, Ginanchyo, Hashima, Gifu 501-61 (Ohohashi), Japan.

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Takahiro Ohohashi From the Laboratories of Veterinary Physiology 1 (Nishita) and Veterinary Anatomy 1 (Asari), School of Veterinary Medicine, Azabu University, 1-17-71, Fuchinobe, Sagamihara, Kanagawa 229, and Ohohashi Veterinary Hospital, 4-49, Yakusiji, Ginanchyo, Hashima, Gifu 501-61 (Ohohashi), Japan.

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Masao Asari From the Laboratories of Veterinary Physiology 1 (Nishita) and Veterinary Anatomy 1 (Asari), School of Veterinary Medicine, Azabu University, 1-17-71, Fuchinobe, Sagamihara, Kanagawa 229, and Ohohashi Veterinary Hospital, 4-49, Yakusiji, Ginanchyo, Hashima, Gifu 501-61 (Ohohashi), Japan.

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SUMMARY

The concentration of carbonic anhydrase III isoenzyme (ca-III) in serum samples from 216 clinically normal Thoroughbreds was determined by use of an enzyme immunoassay. The concentration range of ca-III was from 16.0 to 254.5 ng/ml (mean, 56.5 ± 11.9 ng/ml). Significant differences were not detected according to age or sex. To confirm whether serum ca-III concentration was high in horses with muscle disease, serum samples of 11 horses with exertional rhabdomyolysis were analyzed by enzyme immunoassay. Their serum ca-III concentration was about 56 times (3,136 ± 2,610 ng/ml) that of healthy Thoroughbreds. Concentration of ca-III was higher in horses with rhabdomyolysis that had been transiently recumbent than in horses with mild disease that were reluctant to move. Blood samples obtained serially from 6 horses with exertional rhabdomyolysis were studied. Serum activities of aldolase, creatine kinase, aspartate transaminase, and lactate dehydrogenase were high. Increases and decreases in concentration of ca-III were more rapid than that for aldolase, creatine kinase, aspartate transaminase, and lactate dehydrogenase activities; thus, ca-III may be clinically applicable as a diagnostic marker for muscle disease in horses.

SUMMARY

The concentration of carbonic anhydrase III isoenzyme (ca-III) in serum samples from 216 clinically normal Thoroughbreds was determined by use of an enzyme immunoassay. The concentration range of ca-III was from 16.0 to 254.5 ng/ml (mean, 56.5 ± 11.9 ng/ml). Significant differences were not detected according to age or sex. To confirm whether serum ca-III concentration was high in horses with muscle disease, serum samples of 11 horses with exertional rhabdomyolysis were analyzed by enzyme immunoassay. Their serum ca-III concentration was about 56 times (3,136 ± 2,610 ng/ml) that of healthy Thoroughbreds. Concentration of ca-III was higher in horses with rhabdomyolysis that had been transiently recumbent than in horses with mild disease that were reluctant to move. Blood samples obtained serially from 6 horses with exertional rhabdomyolysis were studied. Serum activities of aldolase, creatine kinase, aspartate transaminase, and lactate dehydrogenase were high. Increases and decreases in concentration of ca-III were more rapid than that for aldolase, creatine kinase, aspartate transaminase, and lactate dehydrogenase activities; thus, ca-III may be clinically applicable as a diagnostic marker for muscle disease in horses.

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