Enzyme-linked immunosorbent assay for screening of milk samples for Salmonella typhimurium in dairy herds

J. Hoorfar From the Danish Veterinary Laboratory, Bülowsvej 27, DK-1790, Copenhagen V (Hoorfar), and Danish Dairy Board, Frederiks Alle 22, DK-8000 Århus C (Wedderkopp), Denmark.

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A. Wedderkopp From the Danish Veterinary Laboratory, Bülowsvej 27, DK-1790, Copenhagen V (Hoorfar), and Danish Dairy Board, Frederiks Alle 22, DK-8000 Århus C (Wedderkopp), Denmark.

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SUMMARY

We investigated the ability of an antibody-specific, O antigen-based elisa to document Salmonella typhimurium herd infections by screening of milk samples. Three cattle populations, 20 herds with no history of salmonellosis, 8 herds with history of S typhimurium epsiodes within the previous 7 months, and 220 herds of unknown disease status, were tested. A herd was considered elisa positive if at least 5% of the cows had OD values > 0.3. Among the 20 herds without history of salmonellosis, only 2 herds were elisa positive, whereas all 8 herds with a known history of salmonellosis were elisa positive (herd specificity, 0.9 and herd sensitivity, 1.0). A significant correlation (P < 0.001) was found between the OD values of serum and milk samples from cows in the herds with a history of salmonellosis. It was concluded that elisa testing of individual milk samples can be used for surveillance of herds for S typhimurium infections, but further modifications are needed to test bulk tank milk samples.

SUMMARY

We investigated the ability of an antibody-specific, O antigen-based elisa to document Salmonella typhimurium herd infections by screening of milk samples. Three cattle populations, 20 herds with no history of salmonellosis, 8 herds with history of S typhimurium epsiodes within the previous 7 months, and 220 herds of unknown disease status, were tested. A herd was considered elisa positive if at least 5% of the cows had OD values > 0.3. Among the 20 herds without history of salmonellosis, only 2 herds were elisa positive, whereas all 8 herds with a known history of salmonellosis were elisa positive (herd specificity, 0.9 and herd sensitivity, 1.0). A significant correlation (P < 0.001) was found between the OD values of serum and milk samples from cows in the herds with a history of salmonellosis. It was concluded that elisa testing of individual milk samples can be used for surveillance of herds for S typhimurium infections, but further modifications are needed to test bulk tank milk samples.

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