Pathogenicity of a bovine attaching effacing Escherichia coli isolate lacking Shiga-like toxins

John Fischer From the Veterinary Medical Diagnostic Laboratory (Fischer, Maddox, Miller) and Department of Veterinary Pathology (Fischer, Kinden, Miller), College of Veterinary Medicine, University of Missouri, Columbia, MO 65211, and Veterinary Diagnostic Center, University of Nebraska, Lincoln, NE 68583 (Moxley).

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Carol Maddox From the Veterinary Medical Diagnostic Laboratory (Fischer, Maddox, Miller) and Department of Veterinary Pathology (Fischer, Kinden, Miller), College of Veterinary Medicine, University of Missouri, Columbia, MO 65211, and Veterinary Diagnostic Center, University of Nebraska, Lincoln, NE 68583 (Moxley).

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Rodney Moxley From the Veterinary Medical Diagnostic Laboratory (Fischer, Maddox, Miller) and Department of Veterinary Pathology (Fischer, Kinden, Miller), College of Veterinary Medicine, University of Missouri, Columbia, MO 65211, and Veterinary Diagnostic Center, University of Nebraska, Lincoln, NE 68583 (Moxley).

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Darrel Kinden From the Veterinary Medical Diagnostic Laboratory (Fischer, Maddox, Miller) and Department of Veterinary Pathology (Fischer, Kinden, Miller), College of Veterinary Medicine, University of Missouri, Columbia, MO 65211, and Veterinary Diagnostic Center, University of Nebraska, Lincoln, NE 68583 (Moxley).

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Margaret Miller From the Veterinary Medical Diagnostic Laboratory (Fischer, Maddox, Miller) and Department of Veterinary Pathology (Fischer, Kinden, Miller), College of Veterinary Medicine, University of Missouri, Columbia, MO 65211, and Veterinary Diagnostic Center, University of Nebraska, Lincoln, NE 68583 (Moxley).

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Summary

Escherichia coli isolate 7996-90, obtained from a calf with diarrhea, had negative results of tests for K-88, K-99, 987P, F41, CS31A, F1845, F165 or E coli adherence factor adhesins and had negative results of tests for the toxins heat-labile, heat-stable A, heatstable B, Shiga-like toxin (slt)-I or slt-II. Strain 7996-90 had localized adherence to HEp-2 cells, caused actin rearrangement in host cells to which it adhered, hybridized with the eaeA probe, and produced the 94-kd outer membrane protein associated with attaching effacing lesions. This isolate caused attaching effacing lesions in Caco-2 cell polar monolayers, rabbit intestinal loops, and the intestines of gnotobiotic pigs. The isolate belongs to serotype O26:NM and is considered a class-II attaching effacing enteropathogenic E coli. Until recent addition of more sensitive assays at veterinary diagnostic laboratories, isolates such as 7996-90 were not readily recognized as pathogens because they failed to fit into the enterohemorrhagic E coli group, members of which, be definition, produce slt. The assays described can facilitate diagnosis of attaching effacing E coli infection when histologic evaluation is hampered by autolysis.

Summary

Escherichia coli isolate 7996-90, obtained from a calf with diarrhea, had negative results of tests for K-88, K-99, 987P, F41, CS31A, F1845, F165 or E coli adherence factor adhesins and had negative results of tests for the toxins heat-labile, heat-stable A, heatstable B, Shiga-like toxin (slt)-I or slt-II. Strain 7996-90 had localized adherence to HEp-2 cells, caused actin rearrangement in host cells to which it adhered, hybridized with the eaeA probe, and produced the 94-kd outer membrane protein associated with attaching effacing lesions. This isolate caused attaching effacing lesions in Caco-2 cell polar monolayers, rabbit intestinal loops, and the intestines of gnotobiotic pigs. The isolate belongs to serotype O26:NM and is considered a class-II attaching effacing enteropathogenic E coli. Until recent addition of more sensitive assays at veterinary diagnostic laboratories, isolates such as 7996-90 were not readily recognized as pathogens because they failed to fit into the enterohemorrhagic E coli group, members of which, be definition, produce slt. The assays described can facilitate diagnosis of attaching effacing E coli infection when histologic evaluation is hampered by autolysis.

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