Influence of estrous cycle stage on adhesion of Streptococcus zooepidemicus to equine endometrium

Graça Ferreira-Dias From the Departments of Physiology (Ferreira-Dias, Nequin) and Animal Science (King), Southern Illinois University, Carbondale, IL 62901.

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Lynn G. Nequin From the Departments of Physiology (Ferreira-Dias, Nequin) and Animal Science (King), Southern Illinois University, Carbondale, IL 62901.

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Sheryl S. King From the Departments of Physiology (Ferreira-Dias, Nequin) and Animal Science (King), Southern Illinois University, Carbondale, IL 62901.

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Summary

Equine endometria representative of Kenney's categories I, II, and III were incubated in vitro with phosphate buffer, Streptococcus pneumoniae, or S zooepidemicus. Endometrial tissues from mares in estrus and diestrus were first categorized according to Kenney's classification, then were tested for adherence of S pneumoniae and S zooepidemicus to the epithelia. Bacteria were not observed when the endometrial tissue was incubated with phosphate buffer or S pneumoniae. There was no statistical difference in attachment of S zooepidemicus to endometrial tissue from mares in estrus or diestrus if endometrial classification was ignored. However, bacterial attachment was significantly (P ≤ 0.05) higher in category III endometrium during estrus.

Summary

Equine endometria representative of Kenney's categories I, II, and III were incubated in vitro with phosphate buffer, Streptococcus pneumoniae, or S zooepidemicus. Endometrial tissues from mares in estrus and diestrus were first categorized according to Kenney's classification, then were tested for adherence of S pneumoniae and S zooepidemicus to the epithelia. Bacteria were not observed when the endometrial tissue was incubated with phosphate buffer or S pneumoniae. There was no statistical difference in attachment of S zooepidemicus to endometrial tissue from mares in estrus or diestrus if endometrial classification was ignored. However, bacterial attachment was significantly (P ≤ 0.05) higher in category III endometrium during estrus.

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