Effect of mimicking prepartum concentration of estradiol-17β on the inflammatory response to endotoxin in gilts

Ulf Magnusson From the Department of Obstetrics and Gynaecology (Magnusson, Kindahl) and the Department of Clinical Chemistry (Holst, Karlsson), Faculty of Veterinary Medicine, Swedish University of Agricultural Sciences, S-75007 Uppsala, Sweden.

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Henrik Holst From the Department of Obstetrics and Gynaecology (Magnusson, Kindahl) and the Department of Clinical Chemistry (Holst, Karlsson), Faculty of Veterinary Medicine, Swedish University of Agricultural Sciences, S-75007 Uppsala, Sweden.

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Hans Kindahl From the Department of Obstetrics and Gynaecology (Magnusson, Kindahl) and the Department of Clinical Chemistry (Holst, Karlsson), Faculty of Veterinary Medicine, Swedish University of Agricultural Sciences, S-75007 Uppsala, Sweden.

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Åsa Karlsson From the Department of Obstetrics and Gynaecology (Magnusson, Kindahl) and the Department of Clinical Chemistry (Holst, Karlsson), Faculty of Veterinary Medicine, Swedish University of Agricultural Sciences, S-75007 Uppsala, Sweden.

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Summary

The effect of mimicking prepartum concentration of estradiol-17β on the inflammatory response to endotoxin in gilts was studied. The study was performed in a split-litter design and comprised 5 pairs of littermates. A catheter was inserted into the jugular vein 2 days prior to the start of the study. In each pair, 1 littermate was treated im with 2.5 mg of estradiol-17β/75 kg of body weight, and the other littermate was given peanut oil im as a control. The day after treatment, all gilts were challenge-exposed with a Salmonella typhimurium-derived endotoxin (1 µg/kg, iv) and the inflammatory response to challenge exposure was monitored. There was no effect of estradiol treatment on the transient clinical signs of endotoxemia or on the increase in rectal temperature. The increase in blood concentrations of prostaglandin F metabolite and cortisol after endotoxin challenge exposure was not affected by estradiol. Decrease in number of circulating blood mononuclear cells and polymorphonuclear leukocytes was not changed by estradiol treatment. Taken together, mimicking prepartum concentration of estradiol did not affect either the magnitude or the kinetics of the inflammatory response to endotoxin in gilts. Relevance of these findings to development of endotoxinmediated diseases, such as the postpartum agalactia syndrome, needs further study.

Summary

The effect of mimicking prepartum concentration of estradiol-17β on the inflammatory response to endotoxin in gilts was studied. The study was performed in a split-litter design and comprised 5 pairs of littermates. A catheter was inserted into the jugular vein 2 days prior to the start of the study. In each pair, 1 littermate was treated im with 2.5 mg of estradiol-17β/75 kg of body weight, and the other littermate was given peanut oil im as a control. The day after treatment, all gilts were challenge-exposed with a Salmonella typhimurium-derived endotoxin (1 µg/kg, iv) and the inflammatory response to challenge exposure was monitored. There was no effect of estradiol treatment on the transient clinical signs of endotoxemia or on the increase in rectal temperature. The increase in blood concentrations of prostaglandin F metabolite and cortisol after endotoxin challenge exposure was not affected by estradiol. Decrease in number of circulating blood mononuclear cells and polymorphonuclear leukocytes was not changed by estradiol treatment. Taken together, mimicking prepartum concentration of estradiol did not affect either the magnitude or the kinetics of the inflammatory response to endotoxin in gilts. Relevance of these findings to development of endotoxinmediated diseases, such as the postpartum agalactia syndrome, needs further study.

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