Identification of subspecies- and serotype 1-specific epitopes on the 80- to 90-kilodalton protein region of Chlamydia psittaci that may be useful for diagnosis of Chlamydial induced abortion

Armel Souriau From Pathologie Infectieuse et Immunologic, INRA Nouzilly 37380, France (Souriau, De Sa, Layachi, Rodolakis) and Department of Patologia, Animal Facultad Veterinaria, Universitad de Murcia, Campus de Espinardo, Murcia, Spain (Salinas).

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Jesus Salinas From Pathologie Infectieuse et Immunologic, INRA Nouzilly 37380, France (Souriau, De Sa, Layachi, Rodolakis) and Department of Patologia, Animal Facultad Veterinaria, Universitad de Murcia, Campus de Espinardo, Murcia, Spain (Salinas).

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Carlos De Sa From Pathologie Infectieuse et Immunologic, INRA Nouzilly 37380, France (Souriau, De Sa, Layachi, Rodolakis) and Department of Patologia, Animal Facultad Veterinaria, Universitad de Murcia, Campus de Espinardo, Murcia, Spain (Salinas).

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Khalid Layachi From Pathologie Infectieuse et Immunologic, INRA Nouzilly 37380, France (Souriau, De Sa, Layachi, Rodolakis) and Department of Patologia, Animal Facultad Veterinaria, Universitad de Murcia, Campus de Espinardo, Murcia, Spain (Salinas).

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Annie Rodolakis From Pathologie Infectieuse et Immunologic, INRA Nouzilly 37380, France (Souriau, De Sa, Layachi, Rodolakis) and Department of Patologia, Animal Facultad Veterinaria, Universitad de Murcia, Campus de Espinardo, Murcia, Spain (Salinas).

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Summary

Genus-, subspecies-, and serotype 1-specific antigens of Chlamydia psittaci were characterized by immunoblot analysis, using monoclonal antibodies that recognize 2 C psittaci strains: AB7 isolated from an ewe that had aborted, and iBl isolated from feces of a healthy ewe. Genus-specific epitopes were detected on lipopolysaccharide, on a 47-kd protein, and on a 27- to 30-kd doublet. Subspecies-specific epitopes were located on a 30-kd protein, and a 80- to 90-kd protein region was identified, which bore subspecies- and serotype l-specific epitopes. These 80- to 90-kd proteins were highly reactive with serum from ewes that had aborted and could be a useful antigen for diagnosis of Chlamydial induced abortion of ruminants.

Summary

Genus-, subspecies-, and serotype 1-specific antigens of Chlamydia psittaci were characterized by immunoblot analysis, using monoclonal antibodies that recognize 2 C psittaci strains: AB7 isolated from an ewe that had aborted, and iBl isolated from feces of a healthy ewe. Genus-specific epitopes were detected on lipopolysaccharide, on a 47-kd protein, and on a 27- to 30-kd doublet. Subspecies-specific epitopes were located on a 30-kd protein, and a 80- to 90-kd protein region was identified, which bore subspecies- and serotype l-specific epitopes. These 80- to 90-kd proteins were highly reactive with serum from ewes that had aborted and could be a useful antigen for diagnosis of Chlamydial induced abortion of ruminants.

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