Evaluation of an orally administered vaccine, using hydrogels containing bacterial exotoxins of Pasteurella haemolytica, in cattle

Terry L. Bowersock From the Schools of Veterinary Medicine (Bowersock, Levy, White, Borie), Pharmacy (Shalaby, Park), and Math (Samuels), Purdue University, West Lafayette, IN 47907-1243, and Brookwood Biomedical Center (Lallone, Leymeyer), Birmingham, AL 35209.

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Waleed S.W. Shalaby From the Schools of Veterinary Medicine (Bowersock, Levy, White, Borie), Pharmacy (Shalaby, Park), and Math (Samuels), Purdue University, West Lafayette, IN 47907-1243, and Brookwood Biomedical Center (Lallone, Leymeyer), Birmingham, AL 35209.

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Michel Levy From the Schools of Veterinary Medicine (Bowersock, Levy, White, Borie), Pharmacy (Shalaby, Park), and Math (Samuels), Purdue University, West Lafayette, IN 47907-1243, and Brookwood Biomedical Center (Lallone, Leymeyer), Birmingham, AL 35209.

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Myra L. Samuels From the Schools of Veterinary Medicine (Bowersock, Levy, White, Borie), Pharmacy (Shalaby, Park), and Math (Samuels), Purdue University, West Lafayette, IN 47907-1243, and Brookwood Biomedical Center (Lallone, Leymeyer), Birmingham, AL 35209.

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Roger Lallone From the Schools of Veterinary Medicine (Bowersock, Levy, White, Borie), Pharmacy (Shalaby, Park), and Math (Samuels), Purdue University, West Lafayette, IN 47907-1243, and Brookwood Biomedical Center (Lallone, Leymeyer), Birmingham, AL 35209.

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M. Randy White From the Schools of Veterinary Medicine (Bowersock, Levy, White, Borie), Pharmacy (Shalaby, Park), and Math (Samuels), Purdue University, West Lafayette, IN 47907-1243, and Brookwood Biomedical Center (Lallone, Leymeyer), Birmingham, AL 35209.

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Della L. Borie From the Schools of Veterinary Medicine (Bowersock, Levy, White, Borie), Pharmacy (Shalaby, Park), and Math (Samuels), Purdue University, West Lafayette, IN 47907-1243, and Brookwood Biomedical Center (Lallone, Leymeyer), Birmingham, AL 35209.

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Joyce Lehmeyer From the Schools of Veterinary Medicine (Bowersock, Levy, White, Borie), Pharmacy (Shalaby, Park), and Math (Samuels), Purdue University, West Lafayette, IN 47907-1243, and Brookwood Biomedical Center (Lallone, Leymeyer), Birmingham, AL 35209.

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Kinam Park From the Schools of Veterinary Medicine (Bowersock, Levy, White, Borie), Pharmacy (Shalaby, Park), and Math (Samuels), Purdue University, West Lafayette, IN 47907-1243, and Brookwood Biomedical Center (Lallone, Leymeyer), Birmingham, AL 35209.

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Summary

Poly(methacrylic acid) hydrogels were tested for oral delivery of a vaccine against Pasteurella haemolytica infection in cattle. Culture supernatants of P haemolytica, the most common bacterium associated with pneumonia in cattle, were used as the antigens in the vaccine. Hydrogels containing culture supernatants were administered orally to calves. Calves were then challenge-exposed with virulent P haemolytica. Calves were euthanatized 3 days after challenge exposure. The lungs of each calf were scored for severity and size of pneumonic lesions. Results indicated that vaccinated calves had smaller, less severe pneumonic lesions and lived longer than nonvaccinated calves. These results indicated that hydrogels can be used to deliver vaccines orally to calves to enhance resistance to pneumonia caused by P haemolytica.

Summary

Poly(methacrylic acid) hydrogels were tested for oral delivery of a vaccine against Pasteurella haemolytica infection in cattle. Culture supernatants of P haemolytica, the most common bacterium associated with pneumonia in cattle, were used as the antigens in the vaccine. Hydrogels containing culture supernatants were administered orally to calves. Calves were then challenge-exposed with virulent P haemolytica. Calves were euthanatized 3 days after challenge exposure. The lungs of each calf were scored for severity and size of pneumonic lesions. Results indicated that vaccinated calves had smaller, less severe pneumonic lesions and lived longer than nonvaccinated calves. These results indicated that hydrogels can be used to deliver vaccines orally to calves to enhance resistance to pneumonia caused by P haemolytica.

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