Increase of mannose residues, as Salmonella typhimurium-adhering factor, on the cecal mucosa of germ-free chickens infected with Eimeria tenella

Eiichiroh Baba From the Department of Veterinary Medicine, College of Agriculture, University of Osaka Prefecture, Gakuencho, Sakai, Osaka 593, Japan

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Yasutaka Tsukamoto From the Department of Veterinary Medicine, College of Agriculture, University of Osaka Prefecture, Gakuencho, Sakai, Osaka 593, Japan

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Tsuneo Fukata From the Department of Veterinary Medicine, College of Agriculture, University of Osaka Prefecture, Gakuencho, Sakai, Osaka 593, Japan

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Kazumi Sasai From the Department of Veterinary Medicine, College of Agriculture, University of Osaka Prefecture, Gakuencho, Sakai, Osaka 593, Japan

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Akira Arakawa From the Department of Veterinary Medicine, College of Agriculture, University of Osaka Prefecture, Gakuencho, Sakai, Osaka 593, Japan

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Summary

To study increase of the Salmonella population in the cecum of chickens infected with Eimeria te-nella, changes in mannose residues on the cecal mucosa were investigated. Inhibition of S typhimurium adherence to the cecum by a 2% carbohydrate (d-mannose, d-galactose, l-fucose, α-methyl- d-glucoside) in phosphate-buffered saline solution was examined. Only d-mannose had inhibitory effects. Whereas d-galactose had somewhat enhancing effects on adherence of S typhimurium to the cecal mucosa of uninfected germ-free chickens. In infected and uninfected chickens, d-mannose inhibited adherence of Styphimurium. d-Mannose significantly (P< 0.05) increased adherence of Bacteroides sp. In infected and uninfected chickens, d-mannose did not have any effect on adherence of Clostridium perfringens and Bifidobacterium thermophilum. Under microscopic observation, only concanavalin A and Lens culinaris agglutinin, of 8 lectins examined, were recognized as lectin-positive staining lines or spots in the cecal mucosa, indicating presence of mannose residues on the cecal mucosa. In E tenella-infected chickens, lectin-positive staining was seen strongly on the coarse surface of damaged cells and at the bottom of the crypts. These results indicate that coccidial infection may induce increase of mannose residues on the intestinal surface and allow adhesion of more salmonellae to the intestine.

Summary

To study increase of the Salmonella population in the cecum of chickens infected with Eimeria te-nella, changes in mannose residues on the cecal mucosa were investigated. Inhibition of S typhimurium adherence to the cecum by a 2% carbohydrate (d-mannose, d-galactose, l-fucose, α-methyl- d-glucoside) in phosphate-buffered saline solution was examined. Only d-mannose had inhibitory effects. Whereas d-galactose had somewhat enhancing effects on adherence of S typhimurium to the cecal mucosa of uninfected germ-free chickens. In infected and uninfected chickens, d-mannose inhibited adherence of Styphimurium. d-Mannose significantly (P< 0.05) increased adherence of Bacteroides sp. In infected and uninfected chickens, d-mannose did not have any effect on adherence of Clostridium perfringens and Bifidobacterium thermophilum. Under microscopic observation, only concanavalin A and Lens culinaris agglutinin, of 8 lectins examined, were recognized as lectin-positive staining lines or spots in the cecal mucosa, indicating presence of mannose residues on the cecal mucosa. In E tenella-infected chickens, lectin-positive staining was seen strongly on the coarse surface of damaged cells and at the bottom of the crypts. These results indicate that coccidial infection may induce increase of mannose residues on the intestinal surface and allow adhesion of more salmonellae to the intestine.

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