Production of monoclonal antibodies specific for antigens derived from tissue of chinook salmon (Oncorhynchus tshawytscha) affected with plasmacytoid leukemia

Garret C. Newbound From the Departments of Pathology and Microbiology (New-bound, Markham, Speare, Després, Horney, Kilbenge, Sheppard) and of Anatomy and Physiology (Wright), Atlantic Veterinary College University of Prince Edward Island, Charlottetown, Prince Edwad Island Canada CIA 4P3, the Department of Population Medicine, Ontario Veterinary College, University of Guelph, Guelph, Ontario N1G 2W1 (Saksida), and the Biological Sciences Branch, Department of Fisheries and Oceans, Pacific Biological Station, Nanaimo, British Columbia V9R 5K6.

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Robert J. F. Markham From the Departments of Pathology and Microbiology (New-bound, Markham, Speare, Després, Horney, Kilbenge, Sheppard) and of Anatomy and Physiology (Wright), Atlantic Veterinary College University of Prince Edward Island, Charlottetown, Prince Edwad Island Canada CIA 4P3, the Department of Population Medicine, Ontario Veterinary College, University of Guelph, Guelph, Ontario N1G 2W1 (Saksida), and the Biological Sciences Branch, Department of Fisheries and Oceans, Pacific Biological Station, Nanaimo, British Columbia V9R 5K6.

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David J. Speare From the Departments of Pathology and Microbiology (New-bound, Markham, Speare, Després, Horney, Kilbenge, Sheppard) and of Anatomy and Physiology (Wright), Atlantic Veterinary College University of Prince Edward Island, Charlottetown, Prince Edwad Island Canada CIA 4P3, the Department of Population Medicine, Ontario Veterinary College, University of Guelph, Guelph, Ontario N1G 2W1 (Saksida), and the Biological Sciences Branch, Department of Fisheries and Oceans, Pacific Biological Station, Nanaimo, British Columbia V9R 5K6.

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Sonja M. Saksida From the Departments of Pathology and Microbiology (New-bound, Markham, Speare, Després, Horney, Kilbenge, Sheppard) and of Anatomy and Physiology (Wright), Atlantic Veterinary College University of Prince Edward Island, Charlottetown, Prince Edwad Island Canada CIA 4P3, the Department of Population Medicine, Ontario Veterinary College, University of Guelph, Guelph, Ontario N1G 2W1 (Saksida), and the Biological Sciences Branch, Department of Fisheries and Oceans, Pacific Biological Station, Nanaimo, British Columbia V9R 5K6.

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Beatrice M. Després From the Departments of Pathology and Microbiology (New-bound, Markham, Speare, Després, Horney, Kilbenge, Sheppard) and of Anatomy and Physiology (Wright), Atlantic Veterinary College University of Prince Edward Island, Charlottetown, Prince Edwad Island Canada CIA 4P3, the Department of Population Medicine, Ontario Veterinary College, University of Guelph, Guelph, Ontario N1G 2W1 (Saksida), and the Biological Sciences Branch, Department of Fisheries and Oceans, Pacific Biological Station, Nanaimo, British Columbia V9R 5K6.

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Barbara S. Horney From the Departments of Pathology and Microbiology (New-bound, Markham, Speare, Després, Horney, Kilbenge, Sheppard) and of Anatomy and Physiology (Wright), Atlantic Veterinary College University of Prince Edward Island, Charlottetown, Prince Edwad Island Canada CIA 4P3, the Department of Population Medicine, Ontario Veterinary College, University of Guelph, Guelph, Ontario N1G 2W1 (Saksida), and the Biological Sciences Branch, Department of Fisheries and Oceans, Pacific Biological Station, Nanaimo, British Columbia V9R 5K6.

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Frederick S. Kibenge From the Departments of Pathology and Microbiology (New-bound, Markham, Speare, Després, Horney, Kilbenge, Sheppard) and of Anatomy and Physiology (Wright), Atlantic Veterinary College University of Prince Edward Island, Charlottetown, Prince Edwad Island Canada CIA 4P3, the Department of Population Medicine, Ontario Veterinary College, University of Guelph, Guelph, Ontario N1G 2W1 (Saksida), and the Biological Sciences Branch, Department of Fisheries and Oceans, Pacific Biological Station, Nanaimo, British Columbia V9R 5K6.

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Judith A. Sheppard From the Departments of Pathology and Microbiology (New-bound, Markham, Speare, Després, Horney, Kilbenge, Sheppard) and of Anatomy and Physiology (Wright), Atlantic Veterinary College University of Prince Edward Island, Charlottetown, Prince Edwad Island Canada CIA 4P3, the Department of Population Medicine, Ontario Veterinary College, University of Guelph, Guelph, Ontario N1G 2W1 (Saksida), and the Biological Sciences Branch, Department of Fisheries and Oceans, Pacific Biological Station, Nanaimo, British Columbia V9R 5K6.

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Glenda M. Wright From the Departments of Pathology and Microbiology (New-bound, Markham, Speare, Després, Horney, Kilbenge, Sheppard) and of Anatomy and Physiology (Wright), Atlantic Veterinary College University of Prince Edward Island, Charlottetown, Prince Edwad Island Canada CIA 4P3, the Department of Population Medicine, Ontario Veterinary College, University of Guelph, Guelph, Ontario N1G 2W1 (Saksida), and the Biological Sciences Branch, Department of Fisheries and Oceans, Pacific Biological Station, Nanaimo, British Columbia V9R 5K6.

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Michael L. Kent From the Departments of Pathology and Microbiology (New-bound, Markham, Speare, Després, Horney, Kilbenge, Sheppard) and of Anatomy and Physiology (Wright), Atlantic Veterinary College University of Prince Edward Island, Charlottetown, Prince Edwad Island Canada CIA 4P3, the Department of Population Medicine, Ontario Veterinary College, University of Guelph, Guelph, Ontario N1G 2W1 (Saksida), and the Biological Sciences Branch, Department of Fisheries and Oceans, Pacific Biological Station, Nanaimo, British Columbia V9R 5K6.

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Summary

Two distinct monoclonal antibodies (mab) were prepared for testing with kidney, spleen, and retrobulae tissue imprints made from chinook salmon (Oncorhnchus tshawytscha) affected with plasmacytoid leukemia. (pl). Hybridomas were prepared from mice immunized with whole and lysed cells purified from renal or retrobular pl-positive tissues, which had been obtained from naturally and experimentally infected fish from British Columbia, Canada. The mab reacted with at least 4 morphologically different cell types; of fluorescence was associated with the plasma membrane and cytoplasm. The mab also reacted with kidney imprints made from chinook salmon affected with a pl-like lymphoproliferative disease in California, indicating that these 2 diseases might be caused by a similar agent. The mab did not react with any of the kidney or spleen imprints made from wild chinook salmon collected from a river in Ontario, Canada

Summary

Two distinct monoclonal antibodies (mab) were prepared for testing with kidney, spleen, and retrobulae tissue imprints made from chinook salmon (Oncorhnchus tshawytscha) affected with plasmacytoid leukemia. (pl). Hybridomas were prepared from mice immunized with whole and lysed cells purified from renal or retrobular pl-positive tissues, which had been obtained from naturally and experimentally infected fish from British Columbia, Canada. The mab reacted with at least 4 morphologically different cell types; of fluorescence was associated with the plasma membrane and cytoplasm. The mab also reacted with kidney imprints made from chinook salmon affected with a pl-like lymphoproliferative disease in California, indicating that these 2 diseases might be caused by a similar agent. The mab did not react with any of the kidney or spleen imprints made from wild chinook salmon collected from a river in Ontario, Canada

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