Serum interleukin-6 concentrations in endotoxin-infused neonatal foals

Julie A. Robinson From the Departments of Veterinary Microbiology (Robinson, Allen), and Veterinary Medicine and Surgery (Green, Garner), College of Veterinary Medicine, the Department of Animal Sciences (Loch), College of Agriculture, Food and Natural Resources, University of Missouri-Columbia, Columbia, MO 65211, and the Homestead Equine Hospital, 3615 Bassett Road, Pacific, MO 63069 (Walsh).

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Gary K. Allen From the Departments of Veterinary Microbiology (Robinson, Allen), and Veterinary Medicine and Surgery (Green, Garner), College of Veterinary Medicine, the Department of Animal Sciences (Loch), College of Agriculture, Food and Natural Resources, University of Missouri-Columbia, Columbia, MO 65211, and the Homestead Equine Hospital, 3615 Bassett Road, Pacific, MO 63069 (Walsh).

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Eleanor M. Green From the Departments of Veterinary Microbiology (Robinson, Allen), and Veterinary Medicine and Surgery (Green, Garner), College of Veterinary Medicine, the Department of Animal Sciences (Loch), College of Agriculture, Food and Natural Resources, University of Missouri-Columbia, Columbia, MO 65211, and the Homestead Equine Hospital, 3615 Bassett Road, Pacific, MO 63069 (Walsh).

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Harold E. Garner From the Departments of Veterinary Microbiology (Robinson, Allen), and Veterinary Medicine and Surgery (Green, Garner), College of Veterinary Medicine, the Department of Animal Sciences (Loch), College of Agriculture, Food and Natural Resources, University of Missouri-Columbia, Columbia, MO 65211, and the Homestead Equine Hospital, 3615 Bassett Road, Pacific, MO 63069 (Walsh).

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Wayne E. Loch From the Departments of Veterinary Microbiology (Robinson, Allen), and Veterinary Medicine and Surgery (Green, Garner), College of Veterinary Medicine, the Department of Animal Sciences (Loch), College of Agriculture, Food and Natural Resources, University of Missouri-Columbia, Columbia, MO 65211, and the Homestead Equine Hospital, 3615 Bassett Road, Pacific, MO 63069 (Walsh).

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Donald M. Walsh From the Departments of Veterinary Microbiology (Robinson, Allen), and Veterinary Medicine and Surgery (Green, Garner), College of Veterinary Medicine, the Department of Animal Sciences (Loch), College of Agriculture, Food and Natural Resources, University of Missouri-Columbia, Columbia, MO 65211, and the Homestead Equine Hospital, 3615 Bassett Road, Pacific, MO 63069 (Walsh).

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Summary

Serum interleukin-6 (il-6) concentration was measured in 11 colostrum-fed (cf) and 8 colostrum-deprived (cd) 2- to 3-day-old foals after foals were infused with lipopolysaccharide (lps; Escherichia coli O55:B5 endotoxin, 0.5 µ.g/kg of body weight in sterile saline [0.9% NaCl] solution). Four cf and 2 cd foals were given saline solution alone. Serum il-6 concentration was estimated by use of an in vitro proliferative bioassay, using the IL-6 dependent B.13.29 clone 9 cells. Interleukin-6 concentration increased in all lps-infused foals, and geometric mean serum il- 6 concentration was significantly higher in cf than cd foals 30 and 90 minutes after infusion. Both lps- infused groups had multiple spikes of mean il-6 concentration that peaked at 120 minutes in cf foals and 150 minutes in cd foals. Results indicated that il-6 is produced in neonatal foals in response to lps infusion. Furthermore, colostrum deprivation resulted in longer times to peak mean serum il-6 concentration and tended to reduce serum il-6 concentration in neonatal foals.

Summary

Serum interleukin-6 (il-6) concentration was measured in 11 colostrum-fed (cf) and 8 colostrum-deprived (cd) 2- to 3-day-old foals after foals were infused with lipopolysaccharide (lps; Escherichia coli O55:B5 endotoxin, 0.5 µ.g/kg of body weight in sterile saline [0.9% NaCl] solution). Four cf and 2 cd foals were given saline solution alone. Serum il-6 concentration was estimated by use of an in vitro proliferative bioassay, using the IL-6 dependent B.13.29 clone 9 cells. Interleukin-6 concentration increased in all lps-infused foals, and geometric mean serum il- 6 concentration was significantly higher in cf than cd foals 30 and 90 minutes after infusion. Both lps- infused groups had multiple spikes of mean il-6 concentration that peaked at 120 minutes in cf foals and 150 minutes in cd foals. Results indicated that il-6 is produced in neonatal foals in response to lps infusion. Furthermore, colostrum deprivation resulted in longer times to peak mean serum il-6 concentration and tended to reduce serum il-6 concentration in neonatal foals.

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