Transmission of proliferative enteritis to swine by use of embryonating chicken eggs

Gary F. Jones From the Department of Veterinary PathoBiology (Jones, Ward, Gebhart) and the Department of Veterinary Diagnostic Medicine (Collins), College of Veterinary Medicine, University of Minnesota, St. Paul, MN 55108.

Search for other papers by Gary F. Jones in
Current site
Google Scholar
PubMed
Close
 DVM
,
Gilbert E. Ward From the Department of Veterinary PathoBiology (Jones, Ward, Gebhart) and the Department of Veterinary Diagnostic Medicine (Collins), College of Veterinary Medicine, University of Minnesota, St. Paul, MN 55108.

Search for other papers by Gilbert E. Ward in
Current site
Google Scholar
PubMed
Close
 DVM, PhD
,
James E. Collins From the Department of Veterinary PathoBiology (Jones, Ward, Gebhart) and the Department of Veterinary Diagnostic Medicine (Collins), College of Veterinary Medicine, University of Minnesota, St. Paul, MN 55108.

Search for other papers by James E. Collins in
Current site
Google Scholar
PubMed
Close
 DVM, PhD
, and
Connie J. Gebhart From the Department of Veterinary PathoBiology (Jones, Ward, Gebhart) and the Department of Veterinary Diagnostic Medicine (Collins), College of Veterinary Medicine, University of Minnesota, St. Paul, MN 55108.

Search for other papers by Connie J. Gebhart in
Current site
Google Scholar
PubMed
Close
 PhD

Click on author name to view affiliation information

Summary

Embryonating eggs were inoculated with filtered porcine ileal mucosa containing intracellular curved rods (icr) and incubated for 4 to 6 days. Three of 12 pigs given the eggs per os developed microscopic lesions of proliferative enteritis (pe). Nonchallenge-exposed control pigs did not develop lesions of pe. Four of six positive control pigs given ileal mucosa from pigs with pe also developed microscopic lesions of pe. All of the PE lesions were found in pigs necropsied 10 to 29 days after challenge exposure. None of the swine in the study had clinical signs or gross lesions of pe.

Campylobacter spp were isolated from pigs with and without exposure to the ileal mucosa from pigs with pe. There was no relationship between Campylobacter spp isolation and development of lesions.

Deoxyribonucleic acids extracted from embryonating chicken eggs injected with the equivalent of 0.5 mg of mucosal lesions and incubated for 4 days hybridized to a dna probe specific for the icr, whereas dna extracted from 1.5 mg of mucosal homogenates of the same proliferative tissue did not hybridize with the same probe. Results of these experiments indicated that icr injected into eggs remained infective for pigs and suggest replication of icr in the first-passage eggs.

Summary

Embryonating eggs were inoculated with filtered porcine ileal mucosa containing intracellular curved rods (icr) and incubated for 4 to 6 days. Three of 12 pigs given the eggs per os developed microscopic lesions of proliferative enteritis (pe). Nonchallenge-exposed control pigs did not develop lesions of pe. Four of six positive control pigs given ileal mucosa from pigs with pe also developed microscopic lesions of pe. All of the PE lesions were found in pigs necropsied 10 to 29 days after challenge exposure. None of the swine in the study had clinical signs or gross lesions of pe.

Campylobacter spp were isolated from pigs with and without exposure to the ileal mucosa from pigs with pe. There was no relationship between Campylobacter spp isolation and development of lesions.

Deoxyribonucleic acids extracted from embryonating chicken eggs injected with the equivalent of 0.5 mg of mucosal lesions and incubated for 4 days hybridized to a dna probe specific for the icr, whereas dna extracted from 1.5 mg of mucosal homogenates of the same proliferative tissue did not hybridize with the same probe. Results of these experiments indicated that icr injected into eggs remained infective for pigs and suggest replication of icr in the first-passage eggs.

All Time Past Year Past 30 Days
Abstract Views 0 0 0
Full Text Views 42 42 11
PDF Downloads 31 31 5
Advertisement