Immunoglobulins in stimulated tears of dogs

Pedro J. Ginel From the Department of Veterinary Clinical Pathology (Ginel, Novales, Martín, López, Molleda), College of Veterinary Medicine, University of Córdoba, and the Department of Immunology (García), The Reína Sofía Hospital, Córdoba, Spain. Dr. Ginel’s present address is Department of Veterinary Clinical Pathology, College of Veterinary Medicine, Medina Azahara 9, 14005 Córdoba, Spain.

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Manuel Novales From the Department of Veterinary Clinical Pathology (Ginel, Novales, Martín, López, Molleda), College of Veterinary Medicine, University of Córdoba, and the Department of Immunology (García), The Reína Sofía Hospital, Córdoba, Spain. Dr. Ginel’s present address is Department of Veterinary Clinical Pathology, College of Veterinary Medicine, Medina Azahara 9, 14005 Córdoba, Spain.

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María García From the Department of Veterinary Clinical Pathology (Ginel, Novales, Martín, López, Molleda), College of Veterinary Medicine, University of Córdoba, and the Department of Immunology (García), The Reína Sofía Hospital, Córdoba, Spain. Dr. Ginel’s present address is Department of Veterinary Clinical Pathology, College of Veterinary Medicine, Medina Azahara 9, 14005 Córdoba, Spain.

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Eva M. Martín From the Department of Veterinary Clinical Pathology (Ginel, Novales, Martín, López, Molleda), College of Veterinary Medicine, University of Córdoba, and the Department of Immunology (García), The Reína Sofía Hospital, Córdoba, Spain. Dr. Ginel’s present address is Department of Veterinary Clinical Pathology, College of Veterinary Medicine, Medina Azahara 9, 14005 Córdoba, Spain.

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Rocío López From the Department of Veterinary Clinical Pathology (Ginel, Novales, Martín, López, Molleda), College of Veterinary Medicine, University of Córdoba, and the Department of Immunology (García), The Reína Sofía Hospital, Córdoba, Spain. Dr. Ginel’s present address is Department of Veterinary Clinical Pathology, College of Veterinary Medicine, Medina Azahara 9, 14005 Córdoba, Spain.

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José M. Molleda From the Department of Veterinary Clinical Pathology (Ginel, Novales, Martín, López, Molleda), College of Veterinary Medicine, University of Córdoba, and the Department of Immunology (García), The Reína Sofía Hospital, Córdoba, Spain. Dr. Ginel’s present address is Department of Veterinary Clinical Pathology, College of Veterinary Medicine, Medina Azahara 9, 14005 Córdoba, Spain.

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Summary

Immunoelectrophoresis and single radial immunodiffusion were used to identify and measure tear immunoglobulin concentrations in 50 healthy dogs. Immunoglobulin A and IgG were detected in all samples analyzed, whereas IgM was not detected in any sample. Mean IgA concentration was 25.28 ± 1.9 mg/dl, adult dogs (> 18 months) having significantly higher mean value. The IgA concentration related to age had significant (P < 0.006) positive correlation; mean IgG concentration was 23.10 ± 1.72 mg/dl. Linear correlation analysis revealed significant (P < 0.0007) correlation coefficient between tear total protein and IgA concentrations. The IgA and IgG concentrations also were significantly (P < 0.0001) correlated when expressed as milligrams per 100 mg of protein. Relation with sex was not established for either immunoglobulin.

Summary

Immunoelectrophoresis and single radial immunodiffusion were used to identify and measure tear immunoglobulin concentrations in 50 healthy dogs. Immunoglobulin A and IgG were detected in all samples analyzed, whereas IgM was not detected in any sample. Mean IgA concentration was 25.28 ± 1.9 mg/dl, adult dogs (> 18 months) having significantly higher mean value. The IgA concentration related to age had significant (P < 0.006) positive correlation; mean IgG concentration was 23.10 ± 1.72 mg/dl. Linear correlation analysis revealed significant (P < 0.0007) correlation coefficient between tear total protein and IgA concentrations. The IgA and IgG concentrations also were significantly (P < 0.0001) correlated when expressed as milligrams per 100 mg of protein. Relation with sex was not established for either immunoglobulin.

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