Use of indirect enzyme-linked immunosorbent assay with hot saline solution extracts of a variant (M—) strain of Brucella canis for diagnosis of brucellosis in dogs

E. M. Mateu-de-Antonio From the Infectious Diseases and Epidemiology Unit, Veterinary Faculty, Autonomous University of Barcelona, 08193 Bellaterra, Barcelona, Spain.

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M. Martin From the Infectious Diseases and Epidemiology Unit, Veterinary Faculty, Autonomous University of Barcelona, 08193 Bellaterra, Barcelona, Spain.

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M. Soler From the Infectious Diseases and Epidemiology Unit, Veterinary Faculty, Autonomous University of Barcelona, 08193 Bellaterra, Barcelona, Spain.

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Summary

An elisa, using hot saline solution extracts (hss) of a less-mucoid variant (M—) strain of Brucella canis as antigen, was developed for detection of antibodies against B canis in dogs. The test was applied to 177 field serum samples previously tested by use of the 2-mercaptoethanol rapid slide agglutination test, 2-mercaptoethanol-tube agglutination test, and agar gel immunodiffusion containing hss and cytoplasmic antigens of B canis. Results indicated that this elisa seems to be highly specific (95.6%) and slightly less sensitive (93.8%). The hss obtained from B canis wild-type RM 6/66 also have been used, but in our study, it seemed to be unsuitable for use in elisa because of the high background values observed for sera with negative test results.

Summary

An elisa, using hot saline solution extracts (hss) of a less-mucoid variant (M—) strain of Brucella canis as antigen, was developed for detection of antibodies against B canis in dogs. The test was applied to 177 field serum samples previously tested by use of the 2-mercaptoethanol rapid slide agglutination test, 2-mercaptoethanol-tube agglutination test, and agar gel immunodiffusion containing hss and cytoplasmic antigens of B canis. Results indicated that this elisa seems to be highly specific (95.6%) and slightly less sensitive (93.8%). The hss obtained from B canis wild-type RM 6/66 also have been used, but in our study, it seemed to be unsuitable for use in elisa because of the high background values observed for sera with negative test results.

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