Detection of Toxoplasma gondii antigen-containing immune complexes in the serum of cats

Michael R. Lappin From the Department of Clinical Sciences, College Veterinary Medicine and Biomedical Sciences, Colorado State University, Fort Collins, CO 80523.

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Suzanne Cayatte From the Department of Clinical Sciences, College Veterinary Medicine and Biomedical Sciences, Colorado State University, Fort Collins, CO 80523.

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Cynthia C. Powell From the Department of Clinical Sciences, College Veterinary Medicine and Biomedical Sciences, Colorado State University, Fort Collins, CO 80523.

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Andrew Gigliotti From the Department of Clinical Sciences, College Veterinary Medicine and Biomedical Sciences, Colorado State University, Fort Collins, CO 80523.

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Christi Cooper From the Department of Clinical Sciences, College Veterinary Medicine and Biomedical Sciences, Colorado State University, Fort Collins, CO 80523.

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Steven M. Roberts From the Department of Clinical Sciences, College Veterinary Medicine and Biomedical Sciences, Colorado State University, Fort Collins, CO 80523.

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SUMMARY

Enzyme-linked immunosorbent assays for the detection of Toxoplasma gondii antigen-containing IgM immune complexes (T gondii-specific IgM-lC) and IgG immune complexes (T gondii-specific IgG-IC) in the serum of cats were developed. Serum from clinically ill, naturally infected cats; healthy, naturally infected cats; and healthy cats experimentally inoculated with T gondii was assayed. All combinations of T gondii-specific IgM, IgG, antigens, IgM-IC and IgG-IC were detected in naturally infected and experimentally infected cats. Clinically ill cats and cats with ocular signs of toxoplasmosis were more likely than healthy cats to have Tgondii-specific IC in serum. It was concluded that T gondii-specific IC form in the serum of cats, may play a role in clinical disease development, and affect the results of Tgondii-specific IgM, IgG, and antigen serologic assays.

SUMMARY

Enzyme-linked immunosorbent assays for the detection of Toxoplasma gondii antigen-containing IgM immune complexes (T gondii-specific IgM-lC) and IgG immune complexes (T gondii-specific IgG-IC) in the serum of cats were developed. Serum from clinically ill, naturally infected cats; healthy, naturally infected cats; and healthy cats experimentally inoculated with T gondii was assayed. All combinations of T gondii-specific IgM, IgG, antigens, IgM-IC and IgG-IC were detected in naturally infected and experimentally infected cats. Clinically ill cats and cats with ocular signs of toxoplasmosis were more likely than healthy cats to have Tgondii-specific IC in serum. It was concluded that T gondii-specific IC form in the serum of cats, may play a role in clinical disease development, and affect the results of Tgondii-specific IgM, IgG, and antigen serologic assays.

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