Effect of deferoxamine-hydroxyethyl pentafraction starch on free, autogenous full-thickness skin grafts in dogs

Giselle Hosgood From the Department of Veterinary Clinical Sciences (Hosgood. Lewis), the Louisiana Veterinary Diagnostic Laboratory and Department of Pathology (Hodgin, Lopez), and the Biodynamics Institute, Basic Sciences College (Church). Louisiana State University. Baton Rouge. LA 70803

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Daniel D. Lewis From the Department of Veterinary Clinical Sciences (Hosgood. Lewis), the Louisiana Veterinary Diagnostic Laboratory and Department of Pathology (Hodgin, Lopez), and the Biodynamics Institute, Basic Sciences College (Church). Louisiana State University. Baton Rouge. LA 70803

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E. Clay Hodgin From the Department of Veterinary Clinical Sciences (Hosgood. Lewis), the Louisiana Veterinary Diagnostic Laboratory and Department of Pathology (Hodgin, Lopez), and the Biodynamics Institute, Basic Sciences College (Church). Louisiana State University. Baton Rouge. LA 70803

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Daniel F. Church From the Department of Veterinary Clinical Sciences (Hosgood. Lewis), the Louisiana Veterinary Diagnostic Laboratory and Department of Pathology (Hodgin, Lopez), and the Biodynamics Institute, Basic Sciences College (Church). Louisiana State University. Baton Rouge. LA 70803

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Mae K. Lopez From the Department of Veterinary Clinical Sciences (Hosgood. Lewis), the Louisiana Veterinary Diagnostic Laboratory and Department of Pathology (Hodgin, Lopez), and the Biodynamics Institute, Basic Sciences College (Church). Louisiana State University. Baton Rouge. LA 70803

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SUMMARY

Free, autogenous, full-thickness skin grafting was performed on 10 dogs; 5 dogs were given an iron chelator, deferoxamine-10% hydroxyethyl pentafraction starch (DEF-HES; 50 mg/kg of body weight, IV), and 5 dogs were given 10% hydroxyethyl pcntafrac-tion starch (HES) in 0.9% saline solution (5 ml/kg, iv). The percentage of viable graft on day 10 was higher, but not significantly so, in DEF-HES-treated dogs (mean ± SD, 72.6 ± 24.8%; median 76.5%) than in HES-treated dogs (mean ± SD, 46.7 ± 34.3%; median, 48.8%). A trend for a positive correlation between the percentage of viable graft (on day 10) and the percentage of original graft area (on day 28) was observed in MES- and DEF-HES-treated dogs; this trend was significant in HES-treated dogs (P = 0.012). Both groups had significant positive correlation between percentage of viable graft on day 10 and percentage of haired skin on day 28 (HES, P = 0.000002; DEF-HES. P = 0.0148). A unique finding in DEF-HES treated dogs was the consistent observation of foamy macrophages in the dermis adjacent to the grafts, in deep subcutaneous tissue below the grafts, and in normal dermis.

SUMMARY

Free, autogenous, full-thickness skin grafting was performed on 10 dogs; 5 dogs were given an iron chelator, deferoxamine-10% hydroxyethyl pentafraction starch (DEF-HES; 50 mg/kg of body weight, IV), and 5 dogs were given 10% hydroxyethyl pcntafrac-tion starch (HES) in 0.9% saline solution (5 ml/kg, iv). The percentage of viable graft on day 10 was higher, but not significantly so, in DEF-HES-treated dogs (mean ± SD, 72.6 ± 24.8%; median 76.5%) than in HES-treated dogs (mean ± SD, 46.7 ± 34.3%; median, 48.8%). A trend for a positive correlation between the percentage of viable graft (on day 10) and the percentage of original graft area (on day 28) was observed in MES- and DEF-HES-treated dogs; this trend was significant in HES-treated dogs (P = 0.012). Both groups had significant positive correlation between percentage of viable graft on day 10 and percentage of haired skin on day 28 (HES, P = 0.000002; DEF-HES. P = 0.0148). A unique finding in DEF-HES treated dogs was the consistent observation of foamy macrophages in the dermis adjacent to the grafts, in deep subcutaneous tissue below the grafts, and in normal dermis.

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