Changes in fluid composition on the serosal surface of jejunum and small colon subjected to venous strangulation obstruction in ponies

Alan J. Ruggles From the Departments of Clinical Studies (Rugglcs. Freeman) and Pathobiology (Acland. FitzSimmons), School of Veterinary Medicine, New Bolton Center, 382 W Street Rd. Kennett Square, PA 19348.

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David E. Freeman From the Departments of Clinical Studies (Rugglcs. Freeman) and Pathobiology (Acland. FitzSimmons), School of Veterinary Medicine, New Bolton Center, 382 W Street Rd. Kennett Square, PA 19348.

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Helen M. Acland From the Departments of Clinical Studies (Rugglcs. Freeman) and Pathobiology (Acland. FitzSimmons), School of Veterinary Medicine, New Bolton Center, 382 W Street Rd. Kennett Square, PA 19348.

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Marie Fitz Simmons From the Departments of Clinical Studies (Rugglcs. Freeman) and Pathobiology (Acland. FitzSimmons), School of Veterinary Medicine, New Bolton Center, 382 W Street Rd. Kennett Square, PA 19348.

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SUMMARY

In 6 anesthetized ponies, 3 segments of jejunum and 3 segments of small colon were isolated from the peritoneal cavity in plastic bags filled with Hanks' balanced salt solution. One jejunal and 1 small colon segment were subjected to venous strangulation obstruction for 3 hours (vso-3), venous strangulation obstruction for 6 hours (vso-6), or a 6-hour sham procedure to control for changes induced by isolation in a plastic bag. Additional segments of jejunum and colon that were not placed in bags served as controls for histologic examination and collagenase measurements. Samples of fluid surrounding the intestine were obtained for chemical analyses, nucleated cell count, aerobic and anaerobic bacteriologic culture, and measurement of collagenase activity. Full-thickness tissue samples were obtained for histologic examination and measurement of collagenase content.

Bacteria did not cross the intestinal wall after 3 and 6 hours of vso, despite severe mucosal lesions in these segments. At 6 hours, Po2 was significantly less and Pco2 was significantly (P<0.05) greater in the fluid surrounding the vso-6 jejunal segments, compared with the sham jejunal segments. The pH was significantly (P<0.05) less in fluid surrounding vso-6 small colon segments, compared with the sham colon segments at 6 hours. For jejunum and small colon, phosphate and lactate concentrations were significantly (P<0.05) greater in vso-6 fluid than in the corresponding sham fluids at 6 hours. Fibrin formed around all vso segments, although fibrinogen was not detected in the surrounding fluid, indicating possible rapid conversion of fibrinogen to fibrin. Fluid collagenase activity increased significantly (P<0.05) in all segments over 6 hours. The preparation used in this study was successful in measuring local changes on the serosal surface of intestine subjected to vso and in isolating segments under study in a sterile environment.

SUMMARY

In 6 anesthetized ponies, 3 segments of jejunum and 3 segments of small colon were isolated from the peritoneal cavity in plastic bags filled with Hanks' balanced salt solution. One jejunal and 1 small colon segment were subjected to venous strangulation obstruction for 3 hours (vso-3), venous strangulation obstruction for 6 hours (vso-6), or a 6-hour sham procedure to control for changes induced by isolation in a plastic bag. Additional segments of jejunum and colon that were not placed in bags served as controls for histologic examination and collagenase measurements. Samples of fluid surrounding the intestine were obtained for chemical analyses, nucleated cell count, aerobic and anaerobic bacteriologic culture, and measurement of collagenase activity. Full-thickness tissue samples were obtained for histologic examination and measurement of collagenase content.

Bacteria did not cross the intestinal wall after 3 and 6 hours of vso, despite severe mucosal lesions in these segments. At 6 hours, Po2 was significantly less and Pco2 was significantly (P<0.05) greater in the fluid surrounding the vso-6 jejunal segments, compared with the sham jejunal segments. The pH was significantly (P<0.05) less in fluid surrounding vso-6 small colon segments, compared with the sham colon segments at 6 hours. For jejunum and small colon, phosphate and lactate concentrations were significantly (P<0.05) greater in vso-6 fluid than in the corresponding sham fluids at 6 hours. Fibrin formed around all vso segments, although fibrinogen was not detected in the surrounding fluid, indicating possible rapid conversion of fibrinogen to fibrin. Fluid collagenase activity increased significantly (P<0.05) in all segments over 6 hours. The preparation used in this study was successful in measuring local changes on the serosal surface of intestine subjected to vso and in isolating segments under study in a sterile environment.

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