Glycoconjugates as components of receptors for Bordetella avium on the tracheal mucosa or turkeys

L. H. Arp From the Department of Veterinary Pathology, College of Veterinary Medicine, Iowa State University, Ames, IA 50011.

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E. L. Huffman From the Department of Veterinary Pathology, College of Veterinary Medicine, Iowa State University, Ames, IA 50011.

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D. H. Hellwig From the Department of Veterinary Pathology, College of Veterinary Medicine, Iowa State University, Ames, IA 50011.

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Summary

Bordetella avium is an important respiratory tract pathogen of turkeys. In common with other pathogenic bordetellae, B avium manifests a tissue tropism for cilia of the respiratory tract epithelium. To determine the molecular characteristics of the host cell receptors for B avium, we used hemagglutination and in vivo adherence assays. Carbohydrates, mucus, sialic acid-specific lectin, and other glycoconjugates were evaluated for their ability to competitively inhibit binding of B avium to host cells. The gangliosides, GD1a and GT1b, completely inhibited hemagglutination, whereas N-acetylneuraminic acid (sialic acid) partially inhibited hemagglutination. Adherence to turkey tracheal mucosa in vivo was significantly (P < 0.01) inhibited by GD1a and GT1b gangliosides, N-acetylneuraminic acid, bovine submaxillary mucin, and horseshoe crab (Limulus polyphemus) lectin. Treatment of the tracheal mucosa with neuraminidase also inhibited adherence of B avium. We conclude that N-acetylneuraminic acid and the gangliosides, GD1a and GT1b, may be important components or the tracheal mucosa receptor for B avium in turkeys.

Summary

Bordetella avium is an important respiratory tract pathogen of turkeys. In common with other pathogenic bordetellae, B avium manifests a tissue tropism for cilia of the respiratory tract epithelium. To determine the molecular characteristics of the host cell receptors for B avium, we used hemagglutination and in vivo adherence assays. Carbohydrates, mucus, sialic acid-specific lectin, and other glycoconjugates were evaluated for their ability to competitively inhibit binding of B avium to host cells. The gangliosides, GD1a and GT1b, completely inhibited hemagglutination, whereas N-acetylneuraminic acid (sialic acid) partially inhibited hemagglutination. Adherence to turkey tracheal mucosa in vivo was significantly (P < 0.01) inhibited by GD1a and GT1b gangliosides, N-acetylneuraminic acid, bovine submaxillary mucin, and horseshoe crab (Limulus polyphemus) lectin. Treatment of the tracheal mucosa with neuraminidase also inhibited adherence of B avium. We conclude that N-acetylneuraminic acid and the gangliosides, GD1a and GT1b, may be important components or the tracheal mucosa receptor for B avium in turkeys.

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