Specific expression of cellular oncogenes c-myc and c-myb in T-cell lines established from three types of bovine lymphosarcomas

Naotaka Ishiguro From the Department of Veterinary Public Health (Ishiguro, Shinagawa), and the Department of Veterinary Pathology (Matsui), Obihiro University of Agriculture and Veterinary Medicine, Obihiro Hokkaido, 080, Japan.

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Takane Matsui From the Department of Veterinary Public Health (Ishiguro, Shinagawa), and the Department of Veterinary Pathology (Matsui), Obihiro University of Agriculture and Veterinary Medicine, Obihiro Hokkaido, 080, Japan.

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Morikazu Shinagawa From the Department of Veterinary Public Health (Ishiguro, Shinagawa), and the Department of Veterinary Pathology (Matsui), Obihiro University of Agriculture and Veterinary Medicine, Obihiro Hokkaido, 080, Japan.

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Summary

Expression of cellular oncogenes in 3 lymphoid cell lines, BTL-PC3 (BoCD2, BoCD4, BoCD8, BoWC1+), BLS1 (BoCD2+, BoCD4, BoCD8, B0WC1+) and BLT2 (BoCD2, BoCD4, BoCD8, BoWC1), which have been established from calf, skin, and thymic types of lymphosarcomas, respectively, were analyzed by dna-rna (northern blot) hybridization. To determine specific expression of oncogenes involved in malignant transformation of the lymphoid cells, cellular rna was isolated from bovine tumor cell lines, BTL-PC3, BLS1, and BLT2, and from Madin Darby bovine kidney cells used as a control for bovine cell lines. The rna was hybridized against 5 viral oncogene probes (v-jun, v-myc, v-erbB, v-erbA and v-fes), 6 human cellular oncogene probes (N-ras, c-Blym-1 c-erbB-2, c-fos, c-myb and c-abl), human p53 tumor suppressor gene, and bovine LDH-A gene probes. Line BTL-PC3 expressed 2.4-kilobase (kb) c-myc and 4.0- and 3.6-kb c-myb transcripts, and line BLT2 expressed a 3.8-kb c-myb transcript, but line BLS1 expressed no message for the oncogenes tested. Specific transcripts of p53 were found in BTL-PC3 and BLT2 lines, but not in BLS1. Madin Darby bovine kidney cell line expressed multiple cellular oncogenes, c-jun, c-myc, and c-fos, and p53 genes. Southern blot hybridization did not reveal abnormal dna rearrangements associated with the expressed oncogenes (c-myc and c-myb) in the 3 bovine tumor lines. From these results and the immunophenotype of the lymphoid cells, it appeared that c-myb specific mRNA were transcribed more often in immature bovine leukemia T cells (calf and thymic types) than in mature T cells from the skin type.

Summary

Expression of cellular oncogenes in 3 lymphoid cell lines, BTL-PC3 (BoCD2, BoCD4, BoCD8, BoWC1+), BLS1 (BoCD2+, BoCD4, BoCD8, B0WC1+) and BLT2 (BoCD2, BoCD4, BoCD8, BoWC1), which have been established from calf, skin, and thymic types of lymphosarcomas, respectively, were analyzed by dna-rna (northern blot) hybridization. To determine specific expression of oncogenes involved in malignant transformation of the lymphoid cells, cellular rna was isolated from bovine tumor cell lines, BTL-PC3, BLS1, and BLT2, and from Madin Darby bovine kidney cells used as a control for bovine cell lines. The rna was hybridized against 5 viral oncogene probes (v-jun, v-myc, v-erbB, v-erbA and v-fes), 6 human cellular oncogene probes (N-ras, c-Blym-1 c-erbB-2, c-fos, c-myb and c-abl), human p53 tumor suppressor gene, and bovine LDH-A gene probes. Line BTL-PC3 expressed 2.4-kilobase (kb) c-myc and 4.0- and 3.6-kb c-myb transcripts, and line BLT2 expressed a 3.8-kb c-myb transcript, but line BLS1 expressed no message for the oncogenes tested. Specific transcripts of p53 were found in BTL-PC3 and BLT2 lines, but not in BLS1. Madin Darby bovine kidney cell line expressed multiple cellular oncogenes, c-jun, c-myc, and c-fos, and p53 genes. Southern blot hybridization did not reveal abnormal dna rearrangements associated with the expressed oncogenes (c-myc and c-myb) in the 3 bovine tumor lines. From these results and the immunophenotype of the lymphoid cells, it appeared that c-myb specific mRNA were transcribed more often in immature bovine leukemia T cells (calf and thymic types) than in mature T cells from the skin type.

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