Evaluation of the microcirculation of the equine jejunum and ascending colon after ischemia and reperfusion

Robin M. Dabareiner From the Marion duPont Scott Equine Medical Center, Virginia-Maryland Regional College of Veterinary Medicine, Virginia Polytechnic Institute and State University, Leesburg, VA 22075 (Dabareiner, Sullins, White) and the Departments of Surgery (Snyder) and Department of Epidemiology and Preventive Medicine (Gardner), School of Veterinary Medicine, University of California, Davis, CA 95616.

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 DVM, MS
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Jack R. Snyder From the Marion duPont Scott Equine Medical Center, Virginia-Maryland Regional College of Veterinary Medicine, Virginia Polytechnic Institute and State University, Leesburg, VA 22075 (Dabareiner, Sullins, White) and the Departments of Surgery (Snyder) and Department of Epidemiology and Preventive Medicine (Gardner), School of Veterinary Medicine, University of California, Davis, CA 95616.

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Kenneth E. Sullins From the Marion duPont Scott Equine Medical Center, Virginia-Maryland Regional College of Veterinary Medicine, Virginia Polytechnic Institute and State University, Leesburg, VA 22075 (Dabareiner, Sullins, White) and the Departments of Surgery (Snyder) and Department of Epidemiology and Preventive Medicine (Gardner), School of Veterinary Medicine, University of California, Davis, CA 95616.

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Nathaniel A. White II From the Marion duPont Scott Equine Medical Center, Virginia-Maryland Regional College of Veterinary Medicine, Virginia Polytechnic Institute and State University, Leesburg, VA 22075 (Dabareiner, Sullins, White) and the Departments of Surgery (Snyder) and Department of Epidemiology and Preventive Medicine (Gardner), School of Veterinary Medicine, University of California, Davis, CA 95616.

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Ian A. Gardner From the Marion duPont Scott Equine Medical Center, Virginia-Maryland Regional College of Veterinary Medicine, Virginia Polytechnic Institute and State University, Leesburg, VA 22075 (Dabareiner, Sullins, White) and the Departments of Surgery (Snyder) and Department of Epidemiology and Preventive Medicine (Gardner), School of Veterinary Medicine, University of California, Davis, CA 95616.

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Summary

Intramural vascular patterns of the jejunum and colon were evaluated during ischemic strangulation obstruction (iso, 70 minutes) and subsequent reperfusion (60 minutes) in 7 adult anesthetized horses. Microvasculature of experimental and control segments was described by comparison of results from microangiography, light microscopy, and scanning electron microscopy of vascular replicas. Experimental and control segments with isolated vascular arcades were removed either immediately after the experimental period or after 60 minutes of reperfusion. Blood was flushed from the vascular system by use of isotonic NaCl, and the segments were divided. Half of each segment was perfused with a modified radiopaque medium for microangiographic evaluation, and half was perfused with dilute methylmethacrylate to create a vascular replica to be studied by scanning electron microscopy. Microangiographic section also were evaluated for histologic changes.

Microvasculature of jejunal control segments and all colon segments was similar to described normal microvasculature of the equine jejunum and ascending colon. In jejunal iso segments, intramural perfusion was redistributed away from the mucosa. In the villi, the central arteriole was short and convoluted and the subepithelial capillaries were not filled. The submucosal vessels and crypt capillaries were congested, compared with those of controls, and the serosal vessels were not filled in the ischemic segments. Histologic grade II-III mucosal lesion was seen in jejunal iso segments. Reperfused jejunal segments had a transmural hyperemic response, and previously unfilled capillaries were observed in all intestinal layers. After reperfusion, the mucosal lesion progressed to grade III-IV and a cellular infiltrate and edema formation were observed in the serosa.

The intramural vasculature of the ischemic and reperfused colon remain unchanged. Minimal histologic damage was observed in the colon after 70 minutes of iso or after 60 minutes of reperfusion.

Summary

Intramural vascular patterns of the jejunum and colon were evaluated during ischemic strangulation obstruction (iso, 70 minutes) and subsequent reperfusion (60 minutes) in 7 adult anesthetized horses. Microvasculature of experimental and control segments was described by comparison of results from microangiography, light microscopy, and scanning electron microscopy of vascular replicas. Experimental and control segments with isolated vascular arcades were removed either immediately after the experimental period or after 60 minutes of reperfusion. Blood was flushed from the vascular system by use of isotonic NaCl, and the segments were divided. Half of each segment was perfused with a modified radiopaque medium for microangiographic evaluation, and half was perfused with dilute methylmethacrylate to create a vascular replica to be studied by scanning electron microscopy. Microangiographic section also were evaluated for histologic changes.

Microvasculature of jejunal control segments and all colon segments was similar to described normal microvasculature of the equine jejunum and ascending colon. In jejunal iso segments, intramural perfusion was redistributed away from the mucosa. In the villi, the central arteriole was short and convoluted and the subepithelial capillaries were not filled. The submucosal vessels and crypt capillaries were congested, compared with those of controls, and the serosal vessels were not filled in the ischemic segments. Histologic grade II-III mucosal lesion was seen in jejunal iso segments. Reperfused jejunal segments had a transmural hyperemic response, and previously unfilled capillaries were observed in all intestinal layers. After reperfusion, the mucosal lesion progressed to grade III-IV and a cellular infiltrate and edema formation were observed in the serosa.

The intramural vasculature of the ischemic and reperfused colon remain unchanged. Minimal histologic damage was observed in the colon after 70 minutes of iso or after 60 minutes of reperfusion.

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