Use of infected cultured cells to compare ultrastructural features of Neospora caninum from dogs and Toxoplasma gondii

D. S. Lindsay From the Department of Pathobiology, College of Veterinary Medicine, Auburn University, AL 36849-5519 (Lindsay, Toivio-Kinnucan, Blagburn); Department of Veterinary Molecular Biology, Montana State University, Bozeman, MT 59717 (Speer) and USDA, ARS, LPSI, Zoonotic Diseases Laboratory, Beltsville, MD 20705 (Dubey).

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C. A. Speer From the Department of Pathobiology, College of Veterinary Medicine, Auburn University, AL 36849-5519 (Lindsay, Toivio-Kinnucan, Blagburn); Department of Veterinary Molecular Biology, Montana State University, Bozeman, MT 59717 (Speer) and USDA, ARS, LPSI, Zoonotic Diseases Laboratory, Beltsville, MD 20705 (Dubey).

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M. A. Toivio-Kinnucan From the Department of Pathobiology, College of Veterinary Medicine, Auburn University, AL 36849-5519 (Lindsay, Toivio-Kinnucan, Blagburn); Department of Veterinary Molecular Biology, Montana State University, Bozeman, MT 59717 (Speer) and USDA, ARS, LPSI, Zoonotic Diseases Laboratory, Beltsville, MD 20705 (Dubey).

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J. P. Dubey From the Department of Pathobiology, College of Veterinary Medicine, Auburn University, AL 36849-5519 (Lindsay, Toivio-Kinnucan, Blagburn); Department of Veterinary Molecular Biology, Montana State University, Bozeman, MT 59717 (Speer) and USDA, ARS, LPSI, Zoonotic Diseases Laboratory, Beltsville, MD 20705 (Dubey).

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B. L. Blagburn From the Department of Pathobiology, College of Veterinary Medicine, Auburn University, AL 36849-5519 (Lindsay, Toivio-Kinnucan, Blagburn); Department of Veterinary Molecular Biology, Montana State University, Bozeman, MT 59717 (Speer) and USDA, ARS, LPSI, Zoonotic Diseases Laboratory, Beltsville, MD 20705 (Dubey).

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SUMMARY

The ultrastructure of tachyzoites of 3 isolates of Neospora caninum from dogs was examined, using transmission electron microscopy of infected cultured cells. Ultrastructure of the 3 isolates was similar. Tachyzoites had a pellicle, 22 subpellicular microtubules, a conoid, anterior and posterior polar rings, 8 to 12 electron-dense rhoptries, numerous micronemes, a single vesicular nucleus, tubular mitochondria, Golgi complexes, ribosomes, endoplasmic reticula, an inactive micropore, electron-dense bodies, lipid bodies, and amylopectin bodies. Most tachyzoites were located adjacent to the host cell nucleus in a parasitophorous vacuole that contained numerous intravacuolar tubules. Tachyzoites divided by endodyogeny. Neospora caninum tissue cysts were not seen. Comparison of N caninum with Toxoplasma gondii tachyzoites indicated that the 2 species can be differentiated on the basis of structure and numbers of rhoptries and numbers and location of micronemes and electron-dense bodies.

SUMMARY

The ultrastructure of tachyzoites of 3 isolates of Neospora caninum from dogs was examined, using transmission electron microscopy of infected cultured cells. Ultrastructure of the 3 isolates was similar. Tachyzoites had a pellicle, 22 subpellicular microtubules, a conoid, anterior and posterior polar rings, 8 to 12 electron-dense rhoptries, numerous micronemes, a single vesicular nucleus, tubular mitochondria, Golgi complexes, ribosomes, endoplasmic reticula, an inactive micropore, electron-dense bodies, lipid bodies, and amylopectin bodies. Most tachyzoites were located adjacent to the host cell nucleus in a parasitophorous vacuole that contained numerous intravacuolar tubules. Tachyzoites divided by endodyogeny. Neospora caninum tissue cysts were not seen. Comparison of N caninum with Toxoplasma gondii tachyzoites indicated that the 2 species can be differentiated on the basis of structure and numbers of rhoptries and numbers and location of micronemes and electron-dense bodies.

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