Cytogenetic characterization of the Madin-Darby bovine kidney cell line

Ewald Kopp From the Institute for Animal Breeding and Genetics (Kopp, Mayr, Schleger), the Institute for Virology (Nowotny), and the Ludwig Boltzmann Institute for Immuno- and Cytogenetic Research (Schleger), Veterinary University Vienna, linke Bahngasse 11, A-1030 Vienna, Austria.

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Norbert Nowotny From the Institute for Animal Breeding and Genetics (Kopp, Mayr, Schleger), the Institute for Virology (Nowotny), and the Ludwig Boltzmann Institute for Immuno- and Cytogenetic Research (Schleger), Veterinary University Vienna, linke Bahngasse 11, A-1030 Vienna, Austria.

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Burkhard Mayr From the Institute for Animal Breeding and Genetics (Kopp, Mayr, Schleger), the Institute for Virology (Nowotny), and the Ludwig Boltzmann Institute for Immuno- and Cytogenetic Research (Schleger), Veterinary University Vienna, linke Bahngasse 11, A-1030 Vienna, Austria.

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Walter Schleger From the Institute for Animal Breeding and Genetics (Kopp, Mayr, Schleger), the Institute for Virology (Nowotny), and the Ludwig Boltzmann Institute for Immuno- and Cytogenetic Research (Schleger), Veterinary University Vienna, linke Bahngasse 11, A-1030 Vienna, Austria.

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SUMMARY

The permanent bovine cell line, Madin-Darby bovine kidney, was cytogenetically analyzed. Chromosomal staining indicated 52 metaphase chromosomes with variations from 49 to 54. The isochromosomes 5, 12, and 13, and centric fusions between chromosomes 1 and 26, 9 and 11, 9 and 24, 17 and 25, 18 and 23, 20 and 24, and 24 and 27 were considered marker chromosomes.

SUMMARY

The permanent bovine cell line, Madin-Darby bovine kidney, was cytogenetically analyzed. Chromosomal staining indicated 52 metaphase chromosomes with variations from 49 to 54. The isochromosomes 5, 12, and 13, and centric fusions between chromosomes 1 and 26, 9 and 11, 9 and 24, 17 and 25, 18 and 23, 20 and 24, and 24 and 27 were considered marker chromosomes.

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