Long-term cannulation of the ovarian vein in mares

Robert L. Linford From the Department of Surgery (Linford) and Reproduction (McCue, Montavon, Lasley), School of Veterinary Medicine, University of California, Davis, CA 95616.

Search for other papers by Robert L. Linford in
Current site
Google Scholar
PubMed
Close
 DVM, PhD
,
Patrick M. McCue From the Department of Surgery (Linford) and Reproduction (McCue, Montavon, Lasley), School of Veterinary Medicine, University of California, Davis, CA 95616.

Search for other papers by Patrick M. McCue in
Current site
Google Scholar
PubMed
Close
 DVM
,
Stephane Montavon From the Department of Surgery (Linford) and Reproduction (McCue, Montavon, Lasley), School of Veterinary Medicine, University of California, Davis, CA 95616.

Search for other papers by Stephane Montavon in
Current site
Google Scholar
PubMed
Close
 DVM
, and
Bill L. Lasley From the Department of Surgery (Linford) and Reproduction (McCue, Montavon, Lasley), School of Veterinary Medicine, University of California, Davis, CA 95616.

Search for other papers by Bill L. Lasley in
Current site
Google Scholar
PubMed
Close
 PhD

SUMMARY

A cannulation technique was developed to collect blood samples from the ovarian vein of mares over an extended period. Ovarian venous cannulae placed in 4 mares remained patent for a mean (± sem) duration of 36.8 (± 6.2) days. During mid-diestrus, concentrations of progesterone in the ovarian vein ipsilateral to the corpus luteum (1,663.8 ± 238.8 ng/ml) were significantly (P < 0.001) higher than concentrations measured in paired samples from the jugular vein (6.1 ± 0.3 ng/ml). Concentration of estradiol in the ovarian vein ranged from a mean of 1,053.2 ± 303.1 pg/ml during diestrus to 3,353.8 ± 1,052.8 pg/ml during estrus, whereas values for 74% of samples collected concurrently from the jugular vein were near or below the sensitivity of the assay (10 pg/ml).

Results of the study indicate that patent long-term ovarian vein cannulation can be achieved in mares. The cannulation technique provides access to important fundamental information on equine reproductive endocrinology, which to our knowledge, has not been available.

SUMMARY

A cannulation technique was developed to collect blood samples from the ovarian vein of mares over an extended period. Ovarian venous cannulae placed in 4 mares remained patent for a mean (± sem) duration of 36.8 (± 6.2) days. During mid-diestrus, concentrations of progesterone in the ovarian vein ipsilateral to the corpus luteum (1,663.8 ± 238.8 ng/ml) were significantly (P < 0.001) higher than concentrations measured in paired samples from the jugular vein (6.1 ± 0.3 ng/ml). Concentration of estradiol in the ovarian vein ranged from a mean of 1,053.2 ± 303.1 pg/ml during diestrus to 3,353.8 ± 1,052.8 pg/ml during estrus, whereas values for 74% of samples collected concurrently from the jugular vein were near or below the sensitivity of the assay (10 pg/ml).

Results of the study indicate that patent long-term ovarian vein cannulation can be achieved in mares. The cannulation technique provides access to important fundamental information on equine reproductive endocrinology, which to our knowledge, has not been available.

Advertisement