Influence of arachidonic acid metabolites and steroids on function of bovine polymorphonuclear neutrophils

Martina Hoedemaker Dr From the Department of Veterinary Biosciences, College of Veterinary Medicine, University of Illinois, Urbana, IL 61801.

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Lisa A. Lund From the Department of Veterinary Biosciences, College of Veterinary Medicine, University of Illinois, Urbana, IL 61801.

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William C. Wagner From the Department of Veterinary Biosciences, College of Veterinary Medicine, University of Illinois, Urbana, IL 61801.

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SUMMARY

Polymorphonuclear neutrophils (pmn) from 4 ovariectomized healthy cows were incubated with 0 (control), 10-8, 10-7, and 10-6M arachidonic acid metabolites of the cyclo- and lipoxygenase pathways for 30 minutes, and with steroids for 2 hours. Immediately after incubation, pmn were subjected to the following function assays: chemotaxis against zymosan-activated serum, chemotaxis against arachidonic acid metabolite or steroid at the doses given (only control pmn were tested), random migration, ingestion of 125I-iododeoxyuridine-labeled Staphylococcus aureus (125I-IdUR-S aureus), iodination of proteins, cytochrome C reduction, antibody-independent and -dependent cell-mediated cytotoxicity (aicc and adcc). Prostaglandin F was chemoattractant and stimulated ingestion of 125I-IdUR-S aureus. Prostaglandin E2 stimulated cytochrome C reduction, whereas prostacyclin inhibited iodination of proteins. Thromboxane B2 stimulated adcc. Leukotriene B4 was chemoattractant for bovine pmn and stimulated random migration and aicc. 5-Hydroxyeicosatetraenoic acid was also chemoattractant, but inhibited ingestion of 125I-IdUR-S aureus. 15-Hydroxyeicosatetraenoic acid was chemoattractant and decreased adcc. Lipoxin A4 stimulated random migration, whereas lipoxin B4 inhibited chemotaxis against zymosan-activated serum, but was chemoattractant and stimulated cytochrome C reduction. 12-Hydroxyhepadecatrienoic acid and 12-hydroxyeicosatetraenoic acid did not influence any of the pmn functions tested. Of the steroids tested, cortisol increased adcc, and progesterone stimulated cytochrome C reduction, but decreased adcc. 17β-Estradiol and estrone were chemoattractant and stimulated cytochrome C reduction. In addition, estrone also stimulated random migration. Those results suggest that eicosanoids and steroids directly influence function of bovine pmn in vitro in a stimulatory or inhibitory manner, or both, and may act as modulators of bovine pmn function in vivo.

SUMMARY

Polymorphonuclear neutrophils (pmn) from 4 ovariectomized healthy cows were incubated with 0 (control), 10-8, 10-7, and 10-6M arachidonic acid metabolites of the cyclo- and lipoxygenase pathways for 30 minutes, and with steroids for 2 hours. Immediately after incubation, pmn were subjected to the following function assays: chemotaxis against zymosan-activated serum, chemotaxis against arachidonic acid metabolite or steroid at the doses given (only control pmn were tested), random migration, ingestion of 125I-iododeoxyuridine-labeled Staphylococcus aureus (125I-IdUR-S aureus), iodination of proteins, cytochrome C reduction, antibody-independent and -dependent cell-mediated cytotoxicity (aicc and adcc). Prostaglandin F was chemoattractant and stimulated ingestion of 125I-IdUR-S aureus. Prostaglandin E2 stimulated cytochrome C reduction, whereas prostacyclin inhibited iodination of proteins. Thromboxane B2 stimulated adcc. Leukotriene B4 was chemoattractant for bovine pmn and stimulated random migration and aicc. 5-Hydroxyeicosatetraenoic acid was also chemoattractant, but inhibited ingestion of 125I-IdUR-S aureus. 15-Hydroxyeicosatetraenoic acid was chemoattractant and decreased adcc. Lipoxin A4 stimulated random migration, whereas lipoxin B4 inhibited chemotaxis against zymosan-activated serum, but was chemoattractant and stimulated cytochrome C reduction. 12-Hydroxyhepadecatrienoic acid and 12-hydroxyeicosatetraenoic acid did not influence any of the pmn functions tested. Of the steroids tested, cortisol increased adcc, and progesterone stimulated cytochrome C reduction, but decreased adcc. 17β-Estradiol and estrone were chemoattractant and stimulated cytochrome C reduction. In addition, estrone also stimulated random migration. Those results suggest that eicosanoids and steroids directly influence function of bovine pmn in vitro in a stimulatory or inhibitory manner, or both, and may act as modulators of bovine pmn function in vivo.

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