Osmotic stress in red blood cells from Beagles with hemolytic anemia

Cynthia A. Pekow From the Departments of Comparative Medicine (Pekow, Maggio-Price), Pharmacology (Hinds, Vincenzi), and Medicine (Hammond), University of Washington, Seattle, WA 98195.

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Thomas R. Hinds From the Departments of Comparative Medicine (Pekow, Maggio-Price), Pharmacology (Hinds, Vincenzi), and Medicine (Hammond), University of Washington, Seattle, WA 98195.

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Lillian Maggio-Price From the Departments of Comparative Medicine (Pekow, Maggio-Price), Pharmacology (Hinds, Vincenzi), and Medicine (Hammond), University of Washington, Seattle, WA 98195.

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William P. Hammond From the Departments of Comparative Medicine (Pekow, Maggio-Price), Pharmacology (Hinds, Vincenzi), and Medicine (Hammond), University of Washington, Seattle, WA 98195.

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Frank F. Vincenzi From the Departments of Comparative Medicine (Pekow, Maggio-Price), Pharmacology (Hinds, Vincenzi), and Medicine (Hammond), University of Washington, Seattle, WA 98195.

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Summary

Red blood cell populations separated by density centrifugation were compared in a dynamic assay of osmotic stress. Red blood cells from Beagles genotypically normal and nonanemic (nonaffected), Beagles with inherited hemolytic anemia (anemic), and Beagles presumed to be carriers of the anemia trait (trait carriers) were examined for rate and extent of swelling after exposure to the ionophore A23187 in a medium containing calcium and potassium chloride. Comparisons were made between rbc populations separated on the basis of density. Significant differences were observed in the rates of cell swelling in rbc populations separated by density between nonaffected and anemic Beagles. The response of rbc from Beagles presumed to carry the anemia trait was similar to that of rbc from nonaffected dogs. One phenotypic expression of this inherited abnormality of rbc in Beagles was an accelerated rate of rbc swelling under osmotic stress, and this swelling response diminished with increasing rbc density.

Summary

Red blood cell populations separated by density centrifugation were compared in a dynamic assay of osmotic stress. Red blood cells from Beagles genotypically normal and nonanemic (nonaffected), Beagles with inherited hemolytic anemia (anemic), and Beagles presumed to be carriers of the anemia trait (trait carriers) were examined for rate and extent of swelling after exposure to the ionophore A23187 in a medium containing calcium and potassium chloride. Comparisons were made between rbc populations separated on the basis of density. Significant differences were observed in the rates of cell swelling in rbc populations separated by density between nonaffected and anemic Beagles. The response of rbc from Beagles presumed to carry the anemia trait was similar to that of rbc from nonaffected dogs. One phenotypic expression of this inherited abnormality of rbc in Beagles was an accelerated rate of rbc swelling under osmotic stress, and this swelling response diminished with increasing rbc density.

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