Effects of lung site and fluid volume on results of bronchoalveolar lavage fluid analysis in horses

Corinne Raphel Sweeney From the Department of Clinical Studies, New Bolton Center, School of Veterinary Medicine, University of Pennsylvania, 382 West Street Road, Kennett Square, PA 19348.

Search for other papers by Corinne Raphel Sweeney in
Current site
Google Scholar
PubMed
Close
 DVM
,
Yves Rossier From the Department of Clinical Studies, New Bolton Center, School of Veterinary Medicine, University of Pennsylvania, 382 West Street Road, Kennett Square, PA 19348.

Search for other papers by Yves Rossier in
Current site
Google Scholar
PubMed
Close
 DMV
,
Ellen L. Ziemer From the Department of Clinical Studies, New Bolton Center, School of Veterinary Medicine, University of Pennsylvania, 382 West Street Road, Kennett Square, PA 19348.

Search for other papers by Ellen L. Ziemer in
Current site
Google Scholar
PubMed
Close
 DVM, MS
, and
Susan Lindborg From the Department of Clinical Studies, New Bolton Center, School of Veterinary Medicine, University of Pennsylvania, 382 West Street Road, Kennett Square, PA 19348.

Search for other papers by Susan Lindborg in
Current site
Google Scholar
PubMed
Close
 BS

Click on author name to view affiliation information

Summary

Bronchoalveolar lavage (bal) fluid was analyzed in healthy horses, using different lavage fluid volumes and lung sites. The only significant difference in the cellular composition of bal fluid between the right and left lungs was the mast cell numbers, which were significantly higher in the left lung. Total cell count ranged from 34 to 330 cells/μl for the right lung and 43 to 330 cells/μl for the left lung. Percentage of neutrophils ranged from 1 to 7% in the right lung and 1 to 5% in the left lung. The small-volume (50 ml) lavage had a greater percentage of neutrophils and a lesser percentage of mast cells in the large-volume (350 ml) lavage. Statistical difference in the composition of bal fluid recovered was not detected between the 3 sequential 100-ml lavages and a single 300-ml lavage, except that macrophages were significantly higher in the 3 sequential 100-ml lavages. Values for bal fluid analysis in healthy horses have varied considerably and this variation is from a failure to adhere to any standard technique for volume of fluid infused.

Summary

Bronchoalveolar lavage (bal) fluid was analyzed in healthy horses, using different lavage fluid volumes and lung sites. The only significant difference in the cellular composition of bal fluid between the right and left lungs was the mast cell numbers, which were significantly higher in the left lung. Total cell count ranged from 34 to 330 cells/μl for the right lung and 43 to 330 cells/μl for the left lung. Percentage of neutrophils ranged from 1 to 7% in the right lung and 1 to 5% in the left lung. The small-volume (50 ml) lavage had a greater percentage of neutrophils and a lesser percentage of mast cells in the large-volume (350 ml) lavage. Statistical difference in the composition of bal fluid recovered was not detected between the 3 sequential 100-ml lavages and a single 300-ml lavage, except that macrophages were significantly higher in the 3 sequential 100-ml lavages. Values for bal fluid analysis in healthy horses have varied considerably and this variation is from a failure to adhere to any standard technique for volume of fluid infused.

All Time Past Year Past 30 Days
Abstract Views 0 0 0
Full Text Views 44 44 15
PDF Downloads 51 51 20
Advertisement