In vivo isolation of Salmonella choleraesuis from porcine neutrophils

Michael B. Roof From NOBL Laboratories Inc, Sioux Center, IA 51250 (Roof), and the Microbiology, Immunology, and Preventive Medicine (Kramer, Roth) and Department of Veterinary Clinical Sciences (Kunesh), Iowa State University, Ames, IA 50011.

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Theodore T. Kramer From NOBL Laboratories Inc, Sioux Center, IA 51250 (Roof), and the Microbiology, Immunology, and Preventive Medicine (Kramer, Roth) and Department of Veterinary Clinical Sciences (Kunesh), Iowa State University, Ames, IA 50011.

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Jerry P. Kunesh From NOBL Laboratories Inc, Sioux Center, IA 51250 (Roof), and the Microbiology, Immunology, and Preventive Medicine (Kramer, Roth) and Department of Veterinary Clinical Sciences (Kunesh), Iowa State University, Ames, IA 50011.

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James A. Roth From NOBL Laboratories Inc, Sioux Center, IA 51250 (Roof), and the Microbiology, Immunology, and Preventive Medicine (Kramer, Roth) and Department of Veterinary Clinical Sciences (Kunesh), Iowa State University, Ames, IA 50011.

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Summary

Seventy-five pigs from 4 facilities were examined for Salmonella choleraesuis by use of bacteriologic culture of feces, blood, wbc (buffy coat), mononuclear leukocytes, and neutrophils. The organism was isolated from 0 of 75 fecal samples, compared with isolation from 39 of 75 purified neutrophil preparations. Of the pigs that did not have Salmonella isolated from feces or blood, but had S choleraesuis isolated from neutrophils, 6 were further examined. These pigs from 2 groups again had culture performed at least 3 successive times to test for repeatability and to determine optimal number of neutrophils required for Salmonella isolation. These same pigs were euthanatized and necropsied. Nineteen tissue specimens from each pig were obtained for culture, but S choleraesuis was isolated only from neutrophil samples. Results indicate that neutrophils may contribute to the carrier state in pigs and should be cultured when attempting to identify S choleraesuis carrier swine.

Summary

Seventy-five pigs from 4 facilities were examined for Salmonella choleraesuis by use of bacteriologic culture of feces, blood, wbc (buffy coat), mononuclear leukocytes, and neutrophils. The organism was isolated from 0 of 75 fecal samples, compared with isolation from 39 of 75 purified neutrophil preparations. Of the pigs that did not have Salmonella isolated from feces or blood, but had S choleraesuis isolated from neutrophils, 6 were further examined. These pigs from 2 groups again had culture performed at least 3 successive times to test for repeatability and to determine optimal number of neutrophils required for Salmonella isolation. These same pigs were euthanatized and necropsied. Nineteen tissue specimens from each pig were obtained for culture, but S choleraesuis was isolated only from neutrophil samples. Results indicate that neutrophils may contribute to the carrier state in pigs and should be cultured when attempting to identify S choleraesuis carrier swine.

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