Fibrinogen-degrading proteins from Haemonchus contortus used to vaccinate sheep

R. J. Boisvenue From the Department of Animal Health Discovery Research, Lilly Research Laboratories, Division of Eli Lilly & Co, PO Box 708, Greenfield, IN 46140 (Bois-venue, Stiff, Tonkinson) and Synergen Inc, Boulder, CO 803401 (Cox, Hageman).

Search for other papers by R. J. Boisvenue in
Current site
Google Scholar
PubMed
Close
 PhD
,
M. I. Stiff From the Department of Animal Health Discovery Research, Lilly Research Laboratories, Division of Eli Lilly & Co, PO Box 708, Greenfield, IN 46140 (Bois-venue, Stiff, Tonkinson) and Synergen Inc, Boulder, CO 803401 (Cox, Hageman).

Search for other papers by M. I. Stiff in
Current site
Google Scholar
PubMed
Close
 PhD
,
L. V. Tonkinson From the Department of Animal Health Discovery Research, Lilly Research Laboratories, Division of Eli Lilly & Co, PO Box 708, Greenfield, IN 46140 (Bois-venue, Stiff, Tonkinson) and Synergen Inc, Boulder, CO 803401 (Cox, Hageman).

Search for other papers by L. V. Tonkinson in
Current site
Google Scholar
PubMed
Close
 PhD
,
G. N. Cox From the Department of Animal Health Discovery Research, Lilly Research Laboratories, Division of Eli Lilly & Co, PO Box 708, Greenfield, IN 46140 (Bois-venue, Stiff, Tonkinson) and Synergen Inc, Boulder, CO 803401 (Cox, Hageman).

Search for other papers by G. N. Cox in
Current site
Google Scholar
PubMed
Close
 PhD
, and
R. Hageman From the Department of Animal Health Discovery Research, Lilly Research Laboratories, Division of Eli Lilly & Co, PO Box 708, Greenfield, IN 46140 (Bois-venue, Stiff, Tonkinson) and Synergen Inc, Boulder, CO 803401 (Cox, Hageman).

Search for other papers by R. Hageman in
Current site
Google Scholar
PubMed
Close
 PhD

Click on author name to view affiliation information

Summary

Sixteen nonsibling sheep, approximately 12 months old, that were raised in a helminth-free environment, were used for 2 protection studies 6 months apart. Sheep were vaccinated weekly for 5 weeks by im injection of fibrinogen-degrading proteins derived from the intestinal tract of adult Haemonchus contortus. Ten days after the last vaccination, sheep were given 2,500 infective H contortus larvae by intraruminal injection. Vaccinated sheep produced specific antibodies, and were protected from the worm challenge. Significant differences in mean fecal worm egg counts for 56 days after worm challenge, in mean numbers of H contortus worms, and female fecundity ratios at necropsy were detected in vaccinated sheep, compared with those in control sheep. These data suggest that the fibrinogen-degrading proteins have a protective role in vaccination of sheep against H contortus.

Summary

Sixteen nonsibling sheep, approximately 12 months old, that were raised in a helminth-free environment, were used for 2 protection studies 6 months apart. Sheep were vaccinated weekly for 5 weeks by im injection of fibrinogen-degrading proteins derived from the intestinal tract of adult Haemonchus contortus. Ten days after the last vaccination, sheep were given 2,500 infective H contortus larvae by intraruminal injection. Vaccinated sheep produced specific antibodies, and were protected from the worm challenge. Significant differences in mean fecal worm egg counts for 56 days after worm challenge, in mean numbers of H contortus worms, and female fecundity ratios at necropsy were detected in vaccinated sheep, compared with those in control sheep. These data suggest that the fibrinogen-degrading proteins have a protective role in vaccination of sheep against H contortus.

All Time Past Year Past 30 Days
Abstract Views 0 0 0
Full Text Views 64 64 10
PDF Downloads 29 29 2
Advertisement