Effects of dialyzable lymph node extracts on lymphoblast proliferative capacity of blood mononuclear cells in cattle with chronic paratuberuclosis

John M. Kreeger From the Veterinary Medical Diagnostic Laboratory, College of Veterinary Medicine, University of Missouri, Columbia, MO 65211 (Kreeger) and the Departments of Veterinary Pathology (Snider) and Veterinary Clinical Sciences (01- cott), School of Veterinary Medicine, Louisiana State University, Baton Rouge, LA 70803.

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Theron G. Snider III From the Veterinary Medical Diagnostic Laboratory, College of Veterinary Medicine, University of Missouri, Columbia, MO 65211 (Kreeger) and the Departments of Veterinary Pathology (Snider) and Veterinary Clinical Sciences (01- cott), School of Veterinary Medicine, Louisiana State University, Baton Rouge, LA 70803.

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Bruce M. Olcott From the Veterinary Medical Diagnostic Laboratory, College of Veterinary Medicine, University of Missouri, Columbia, MO 65211 (Kreeger) and the Departments of Veterinary Pathology (Snider) and Veterinary Clinical Sciences (01- cott), School of Veterinary Medicine, Louisiana State University, Baton Rouge, LA 70803.

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Summary

Dialyzable lymph node extracts (dle) containing transfer factor prepared from calves sensitized to Mycobacterium paratuberculosis and keyhole-limpet hemocyanin (klh) were administered to 4 adult cows with chronic paratuberculosis. Cutaneous delayed hypersensitivity, lymphocyte blastogenesis, monocyte migration-inhibition, and lymphoblast proliferative capacity as a reflection of interleukin-2 (il-2) activity were measured in 2 response to M bovis purified protein derivative, johnin, and klh before and after treatment with DLE. Change in cutaneous delayed hypersensitivity was not evident after DLE treatment. Alterations in histologic features of pre- and posttreatment sections of ileum and mesenteric lymph nodes were not detected. Lymph node extract treatment significantly (P < 0.05) increased il-2 activity and migration-inhibition in response to johnin and klh in vitro. Treatment had no effect on lymphocyte blastogenesis. The data indicate that cattle with chronic paratuberculosis may benefit from dle treatment, by virtue of increased il-2 activity, and that effects of dle are at least partially mediated by an increase in il-2 activity.

Summary

Dialyzable lymph node extracts (dle) containing transfer factor prepared from calves sensitized to Mycobacterium paratuberculosis and keyhole-limpet hemocyanin (klh) were administered to 4 adult cows with chronic paratuberculosis. Cutaneous delayed hypersensitivity, lymphocyte blastogenesis, monocyte migration-inhibition, and lymphoblast proliferative capacity as a reflection of interleukin-2 (il-2) activity were measured in 2 response to M bovis purified protein derivative, johnin, and klh before and after treatment with DLE. Change in cutaneous delayed hypersensitivity was not evident after DLE treatment. Alterations in histologic features of pre- and posttreatment sections of ileum and mesenteric lymph nodes were not detected. Lymph node extract treatment significantly (P < 0.05) increased il-2 activity and migration-inhibition in response to johnin and klh in vitro. Treatment had no effect on lymphocyte blastogenesis. The data indicate that cattle with chronic paratuberculosis may benefit from dle treatment, by virtue of increased il-2 activity, and that effects of dle are at least partially mediated by an increase in il-2 activity.

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