Intestinal permeability in pigs during rotavirus infection

Liebe Vellenga From the Clinic for Large Animal Medicine (Vellenga, Wensing, Breukink) and the Department of Veterinary Pathology (Egberts, van Dijk, Mouwen), State University of Utrecht, Yalelaan 16, 3584 TD Utrecht, The Netherlands.

Search for other papers by Liebe Vellenga in
Current site
Google Scholar
PubMed
Close
 DVM, PhD
,
Herman J. A. Egberts From the Clinic for Large Animal Medicine (Vellenga, Wensing, Breukink) and the Department of Veterinary Pathology (Egberts, van Dijk, Mouwen), State University of Utrecht, Yalelaan 16, 3584 TD Utrecht, The Netherlands.

Search for other papers by Herman J. A. Egberts in
Current site
Google Scholar
PubMed
Close
 DVM, PhD
,
Theo Wensing From the Clinic for Large Animal Medicine (Vellenga, Wensing, Breukink) and the Department of Veterinary Pathology (Egberts, van Dijk, Mouwen), State University of Utrecht, Yalelaan 16, 3584 TD Utrecht, The Netherlands.

Search for other papers by Theo Wensing in
Current site
Google Scholar
PubMed
Close
 PhD
,
Jaap E. van Dijk From the Clinic for Large Animal Medicine (Vellenga, Wensing, Breukink) and the Department of Veterinary Pathology (Egberts, van Dijk, Mouwen), State University of Utrecht, Yalelaan 16, 3584 TD Utrecht, The Netherlands.

Search for other papers by Jaap E. van Dijk in
Current site
Google Scholar
PubMed
Close
 DVM, PhD
,
Johan M. V. M. Mouwen From the Clinic for Large Animal Medicine (Vellenga, Wensing, Breukink) and the Department of Veterinary Pathology (Egberts, van Dijk, Mouwen), State University of Utrecht, Yalelaan 16, 3584 TD Utrecht, The Netherlands.

Search for other papers by Johan M. V. M. Mouwen in
Current site
Google Scholar
PubMed
Close
 DVM, PhD
, and
Henk J. Breukink From the Clinic for Large Animal Medicine (Vellenga, Wensing, Breukink) and the Department of Veterinary Pathology (Egberts, van Dijk, Mouwen), State University of Utrecht, Yalelaan 16, 3584 TD Utrecht, The Netherlands.

Search for other papers by Henk J. Breukink in
Current site
Google Scholar
PubMed
Close
 DVM, PhD

Click on author name to view affiliation information

Summary

Macromolecular permeability of the small intestine was tested in four 3-week-old gnotobiotic pigs inoculated with porcine rotavirus strain RV277 (group A). Pigs were administered 126I-labeled polyvinylpyrrolidone (molecular weight [mol wt], 40,000) orally 1 day before and 2 and 24 hours after virus inoculation, and blood samples were obtained every 6 hours. Eight hours after rotavirus inoculation, pigs had watery diarrhea. Increased permeation of 125I-labeled polyvinylpyrrolidone was not observed after clinical signs of infection had developed. Serum total protein and urea nitrogen concentrations increased slightly at the end of the study, probably as a consequence of dehydration. Differences in blood glucose concentration were not seen. At 48 hours after viral inoculation, macromolecular permeability was tested morphologically by injecting horseradish peroxidase (mol wt, 40,000) into the jejunal lumen just distally to the ligamentum colicoduo-denale. After am incubation period of 20 minutes, small segments of jejunum were obtained for stereomicroscopic, histologic, and ultrastructural investigations. Moderate hyperregenerative villus atrophy was found. Ultrastructural changes of the villus epithelium were minor, and increased macromolecular permeation was not observed.

Summary

Macromolecular permeability of the small intestine was tested in four 3-week-old gnotobiotic pigs inoculated with porcine rotavirus strain RV277 (group A). Pigs were administered 126I-labeled polyvinylpyrrolidone (molecular weight [mol wt], 40,000) orally 1 day before and 2 and 24 hours after virus inoculation, and blood samples were obtained every 6 hours. Eight hours after rotavirus inoculation, pigs had watery diarrhea. Increased permeation of 125I-labeled polyvinylpyrrolidone was not observed after clinical signs of infection had developed. Serum total protein and urea nitrogen concentrations increased slightly at the end of the study, probably as a consequence of dehydration. Differences in blood glucose concentration were not seen. At 48 hours after viral inoculation, macromolecular permeability was tested morphologically by injecting horseradish peroxidase (mol wt, 40,000) into the jejunal lumen just distally to the ligamentum colicoduo-denale. After am incubation period of 20 minutes, small segments of jejunum were obtained for stereomicroscopic, histologic, and ultrastructural investigations. Moderate hyperregenerative villus atrophy was found. Ultrastructural changes of the villus epithelium were minor, and increased macromolecular permeation was not observed.

All Time Past Year Past 30 Days
Abstract Views 0 0 0
Full Text Views 69 69 19
PDF Downloads 6 6 0
Advertisement