Effects of equine infectious anemia virus on hematopoietic progenitors in vitro

Christine J. Swardson From the Department of Veterinary Pathobiology, College of Veterinary Medicine, The Ohio State University, Columbus, OH 43210 (Swardson, Kociba), and the Department of Veterinary Microbiology and Pathology, Washington State University, Pullman, WA 99164 (Perryman).

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Gary J. Kociba From the Department of Veterinary Pathobiology, College of Veterinary Medicine, The Ohio State University, Columbus, OH 43210 (Swardson, Kociba), and the Department of Veterinary Microbiology and Pathology, Washington State University, Pullman, WA 99164 (Perryman).

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Lance E. Perryman From the Department of Veterinary Pathobiology, College of Veterinary Medicine, The Ohio State University, Columbus, OH 43210 (Swardson, Kociba), and the Department of Veterinary Microbiology and Pathology, Washington State University, Pullman, WA 99164 (Perryman).

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Summary

Direct effects of equine infectious anemia virus (eiav) on hematopoiesis in vitro were studied. Bone marrow mononuclear cells from clinically normal horses were incubated with 100 TCID50 of ELAV/107 cells. These cells were cultured to assay for colonies derived from erythroid pro-genitors, granulocyte/monocyte progenitors, and fibroblastic progenitors. The eiav had a selective suppressive effect on the erythroid progenitors. Colony-forming units-erythroid were suppressed to 80% of that for medium controls (P = 0.011). Burst-forming units-erythroid were suppressed to 70% of that for medium controls (P = 0.003). Significant effect was not apparent on colony-forming units-granulocyte/macrophage or on colony-forming units-fibroblastic.

Summary

Direct effects of equine infectious anemia virus (eiav) on hematopoiesis in vitro were studied. Bone marrow mononuclear cells from clinically normal horses were incubated with 100 TCID50 of ELAV/107 cells. These cells were cultured to assay for colonies derived from erythroid pro-genitors, granulocyte/monocyte progenitors, and fibroblastic progenitors. The eiav had a selective suppressive effect on the erythroid progenitors. Colony-forming units-erythroid were suppressed to 80% of that for medium controls (P = 0.011). Burst-forming units-erythroid were suppressed to 70% of that for medium controls (P = 0.003). Significant effect was not apparent on colony-forming units-granulocyte/macrophage or on colony-forming units-fibroblastic.

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