Polypeptides associated with Pasteurella multocida infection in rabbits

Thomas E. Zimmerman From the Department of Comparative Medicine, School of Medicine, University of Washington, Seattle, WA 98195.

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 DVM, MPVM
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Barbara J. Deeb From the Department of Comparative Medicine, School of Medicine, University of Washington, Seattle, WA 98195.

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 DVM, MS
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Ronald F. DiGiacomo From the Department of Comparative Medicine, School of Medicine, University of Washington, Seattle, WA 98195.

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 VMD, MPH

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Summary

Polypeptides from whole cell preparations of Pasteurella multocida serotypes A: 12 and A:3 were separated by sodium dodecyl sulfate-polyacrylamide gel electrophoresis and transferred to nitrocellulose paper. Antigens were detected by immunoblot analysis, using sera from 3 groups of rabbits. Sera were obtained from rabbits inoculated intranasally with P multocida serotype A: 12 or A:3, from rabbits maintained in a rabbitry with enzootic P multocida A: 12 infection, and from rabbits maintained in a rabbitry with enzootic P multocida A:3 infection. Immunoblot analyses of pre- and postinoculation sera from experimentally infected rabbits, using serotype A: 12 antigen, revealed 3 polypeptides with approximate molecular mass of 28, 30, and 37 kDa that consistently detected antibodies after P multocida-induced infection. Sera from rabbits naturally infected with either serotype, tested against serotype A: 12 and A:3 antigens, detected the same polypeptides in both serotypes. Thus, immunologic reactivity to these polypeptides may be useful for serologic detection of P multocida infection.

Summary

Polypeptides from whole cell preparations of Pasteurella multocida serotypes A: 12 and A:3 were separated by sodium dodecyl sulfate-polyacrylamide gel electrophoresis and transferred to nitrocellulose paper. Antigens were detected by immunoblot analysis, using sera from 3 groups of rabbits. Sera were obtained from rabbits inoculated intranasally with P multocida serotype A: 12 or A:3, from rabbits maintained in a rabbitry with enzootic P multocida A: 12 infection, and from rabbits maintained in a rabbitry with enzootic P multocida A:3 infection. Immunoblot analyses of pre- and postinoculation sera from experimentally infected rabbits, using serotype A: 12 antigen, revealed 3 polypeptides with approximate molecular mass of 28, 30, and 37 kDa that consistently detected antibodies after P multocida-induced infection. Sera from rabbits naturally infected with either serotype, tested against serotype A: 12 and A:3 antigens, detected the same polypeptides in both serotypes. Thus, immunologic reactivity to these polypeptides may be useful for serologic detection of P multocida infection.

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