Purification of a protein from serum of cattle with hepatic lipidosis, and identification of the protein as haptoglobin

Kazuhiro Yoshino From the Department of Veterinary Biochemistry (Yoshino, Yuasa) and Veterinary Internal Medicine (Takahashi), Rakuno Gakuen University, Ebetsu 069, and Hokkaido Branch Laboratory, National Institute of Animal Health (Katoh), Hitsujigaoka 4, Sapporo 062, Japan.

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Norio Katoh From the Department of Veterinary Biochemistry (Yoshino, Yuasa) and Veterinary Internal Medicine (Takahashi), Rakuno Gakuen University, Ebetsu 069, and Hokkaido Branch Laboratory, National Institute of Animal Health (Katoh), Hitsujigaoka 4, Sapporo 062, Japan.

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Kiyoshi Takahashi From the Department of Veterinary Biochemistry (Yoshino, Yuasa) and Veterinary Internal Medicine (Takahashi), Rakuno Gakuen University, Ebetsu 069, and Hokkaido Branch Laboratory, National Institute of Animal Health (Katoh), Hitsujigaoka 4, Sapporo 062, Japan.

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Akira Yuasa From the Department of Veterinary Biochemistry (Yoshino, Yuasa) and Veterinary Internal Medicine (Takahashi), Rakuno Gakuen University, Ebetsu 069, and Hokkaido Branch Laboratory, National Institute of Animal Health (Katoh), Hitsujigaoka 4, Sapporo 062, Japan.

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Summary

A protein that has 2 subunits with molecular weight of 35,000 and 23,000 was detected in serum of cattle with hepatic lipidosis (fatty liver). The protein was purified from serum obtained from a cow with fatty liver, and was identified as haptoglobin, which is known to have hemoglobin-binding capacity and to be an acute-phase protein. To assess the relevance of haptoglobin in fatty liver, cattle were classified in 3 groups (healthy control, haptoglobin-positive, and haptoglobin-negative); liver triglyceride content and several serum biochemical variables were evaluated for the 3 groups. Compared with the control and haptoglobin-negative cattle, haptoglobin-positive cattle had significantly (P < 0.01) higher liver triglyceride content, serum bilirubin concentration, and aspartate transaminase activity. Serum haptoglobin concentration was high in slaughter cattle (27 of 40 cattle tested), particularly in cows (20/28).

Summary

A protein that has 2 subunits with molecular weight of 35,000 and 23,000 was detected in serum of cattle with hepatic lipidosis (fatty liver). The protein was purified from serum obtained from a cow with fatty liver, and was identified as haptoglobin, which is known to have hemoglobin-binding capacity and to be an acute-phase protein. To assess the relevance of haptoglobin in fatty liver, cattle were classified in 3 groups (healthy control, haptoglobin-positive, and haptoglobin-negative); liver triglyceride content and several serum biochemical variables were evaluated for the 3 groups. Compared with the control and haptoglobin-negative cattle, haptoglobin-positive cattle had significantly (P < 0.01) higher liver triglyceride content, serum bilirubin concentration, and aspartate transaminase activity. Serum haptoglobin concentration was high in slaughter cattle (27 of 40 cattle tested), particularly in cows (20/28).

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