Determination of total protein concentration and viscosity of synovial fluid from the tibiotarsal joints of horses

Nancy L. Korenek From the Departments of Rural Practice (Korenek, Andrews, Faulk) and Pathobiology (Maddux), College of Veterinary Medicine, and Statistical and Computing Services, Agricultural Experiment Station (Sanders), University of Tennessee, PO Box 1071, Knoxville, TN 37901-1071.

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Frank M. Andrews From the Departments of Rural Practice (Korenek, Andrews, Faulk) and Pathobiology (Maddux), College of Veterinary Medicine, and Statistical and Computing Services, Agricultural Experiment Station (Sanders), University of Tennessee, PO Box 1071, Knoxville, TN 37901-1071.

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Jeanne M. Maddux From the Departments of Rural Practice (Korenek, Andrews, Faulk) and Pathobiology (Maddux), College of Veterinary Medicine, and Statistical and Computing Services, Agricultural Experiment Station (Sanders), University of Tennessee, PO Box 1071, Knoxville, TN 37901-1071.

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William L. Sanders From the Departments of Rural Practice (Korenek, Andrews, Faulk) and Pathobiology (Maddux), College of Veterinary Medicine, and Statistical and Computing Services, Agricultural Experiment Station (Sanders), University of Tennessee, PO Box 1071, Knoxville, TN 37901-1071.

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David L. Faulk From the Departments of Rural Practice (Korenek, Andrews, Faulk) and Pathobiology (Maddux), College of Veterinary Medicine, and Statistical and Computing Services, Agricultural Experiment Station (Sanders), University of Tennessee, PO Box 1071, Knoxville, TN 37901-1071.

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Summary

Viscosity of synovial fluid (sf) from 29 clinicadly normal horses was determined by use of a rotational cone and plate microviscosimeter. Total protein concentration in the sf of the 29 horses, as measured with a refractometer, was < 2.5 g/dl. When the Coomassie brilliant blue test was used to determine total protein concentration in sf for 15 horses, the mean value was 1,088 mg/dl. Viscosity values at 60, 30, 12, 6, 3, and 1.5 revolutions/min (rpm) spindle speed were 4.41 ± 1.54 centipoise (cp), 5.29 ± 1.94 cp, 6.76 ± 2.76 cp, 8.52 ± 4.27 cp, 10.41 ± 6.30 cp, and 13.07 ± 9.05 cp, respectively.

Synovial fluid viscosity increased with decreasing rpm and shear rate, but the shape of the curve for each horse fitted the asymptotic curve.

The rotational cone and plate microviscosimeter was an accurate instrument in measuring sf viscosity at multiple rpm or shear rates in horses. The values obtained on clinically normal horses in this study will serve as a baseline for comparison in the evaluation of horses with joint disease.

Summary

Viscosity of synovial fluid (sf) from 29 clinicadly normal horses was determined by use of a rotational cone and plate microviscosimeter. Total protein concentration in the sf of the 29 horses, as measured with a refractometer, was < 2.5 g/dl. When the Coomassie brilliant blue test was used to determine total protein concentration in sf for 15 horses, the mean value was 1,088 mg/dl. Viscosity values at 60, 30, 12, 6, 3, and 1.5 revolutions/min (rpm) spindle speed were 4.41 ± 1.54 centipoise (cp), 5.29 ± 1.94 cp, 6.76 ± 2.76 cp, 8.52 ± 4.27 cp, 10.41 ± 6.30 cp, and 13.07 ± 9.05 cp, respectively.

Synovial fluid viscosity increased with decreasing rpm and shear rate, but the shape of the curve for each horse fitted the asymptotic curve.

The rotational cone and plate microviscosimeter was an accurate instrument in measuring sf viscosity at multiple rpm or shear rates in horses. The values obtained on clinically normal horses in this study will serve as a baseline for comparison in the evaluation of horses with joint disease.

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