Correlation of serum concentration of α1,-acid glycoprotein with lymphocyte blastogenesis and development of experimentally induced or naturally acquired hepatic abscesses in cattle

Yoshiko Motoi From the Feed Safety Division (Motoi) and Systematic Diagnosis Research Division (Oohashi, Nagasawa), National Institute of Animal Health (NIAH), 3-1-1 Kannondai Tsukuba, Ibaraki 305; Tohoku Branch Laboratory of NIAH, Shichinohe Aomori 039-25 (Miyamoto); Morioka Livestock Hygiene Service Center, Takizawa Iwate 020-1 (Itoh); and Saikin Kagaku Institute Co, 5-12 Aoba-ku Hayamacho, Sendai 981, Japan (Tamura).

Search for other papers by Yoshiko Motoi in
Current site
Google Scholar
PubMed
Close
 DVM, PhD
,
Hiroshi Itoh From the Feed Safety Division (Motoi) and Systematic Diagnosis Research Division (Oohashi, Nagasawa), National Institute of Animal Health (NIAH), 3-1-1 Kannondai Tsukuba, Ibaraki 305; Tohoku Branch Laboratory of NIAH, Shichinohe Aomori 039-25 (Miyamoto); Morioka Livestock Hygiene Service Center, Takizawa Iwate 020-1 (Itoh); and Saikin Kagaku Institute Co, 5-12 Aoba-ku Hayamacho, Sendai 981, Japan (Tamura).

Search for other papers by Hiroshi Itoh in
Current site
Google Scholar
PubMed
Close
 DVM
,
Keiji Tamura From the Feed Safety Division (Motoi) and Systematic Diagnosis Research Division (Oohashi, Nagasawa), National Institute of Animal Health (NIAH), 3-1-1 Kannondai Tsukuba, Ibaraki 305; Tohoku Branch Laboratory of NIAH, Shichinohe Aomori 039-25 (Miyamoto); Morioka Livestock Hygiene Service Center, Takizawa Iwate 020-1 (Itoh); and Saikin Kagaku Institute Co, 5-12 Aoba-ku Hayamacho, Sendai 981, Japan (Tamura).

Search for other papers by Keiji Tamura in
Current site
Google Scholar
PubMed
Close
 DVM, PhD
,
Toru Miyamoto From the Feed Safety Division (Motoi) and Systematic Diagnosis Research Division (Oohashi, Nagasawa), National Institute of Animal Health (NIAH), 3-1-1 Kannondai Tsukuba, Ibaraki 305; Tohoku Branch Laboratory of NIAH, Shichinohe Aomori 039-25 (Miyamoto); Morioka Livestock Hygiene Service Center, Takizawa Iwate 020-1 (Itoh); and Saikin Kagaku Institute Co, 5-12 Aoba-ku Hayamacho, Sendai 981, Japan (Tamura).

Search for other papers by Toru Miyamoto in
Current site
Google Scholar
PubMed
Close
 DAS
,
Tsutai Oohashi From the Feed Safety Division (Motoi) and Systematic Diagnosis Research Division (Oohashi, Nagasawa), National Institute of Animal Health (NIAH), 3-1-1 Kannondai Tsukuba, Ibaraki 305; Tohoku Branch Laboratory of NIAH, Shichinohe Aomori 039-25 (Miyamoto); Morioka Livestock Hygiene Service Center, Takizawa Iwate 020-1 (Itoh); and Saikin Kagaku Institute Co, 5-12 Aoba-ku Hayamacho, Sendai 981, Japan (Tamura).

Search for other papers by Tsutai Oohashi in
Current site
Google Scholar
PubMed
Close
 BS
, and
Shigeyoshi Nagasawa From the Feed Safety Division (Motoi) and Systematic Diagnosis Research Division (Oohashi, Nagasawa), National Institute of Animal Health (NIAH), 3-1-1 Kannondai Tsukuba, Ibaraki 305; Tohoku Branch Laboratory of NIAH, Shichinohe Aomori 039-25 (Miyamoto); Morioka Livestock Hygiene Service Center, Takizawa Iwate 020-1 (Itoh); and Saikin Kagaku Institute Co, 5-12 Aoba-ku Hayamacho, Sendai 981, Japan (Tamura).

Search for other papers by Shigeyoshi Nagasawa in
Current site
Google Scholar
PubMed
Close
 DAS, PhD

Click on author name to view affiliation information

Summary

Changes in serum alpha1-acid glycoprotein (α1 AG) concentration in cattle with hepatic abscesses were observed, and function of α1 ag was evaluated, particularly its influence on cellular immune response. Test cattle (n = 4) were inoculated with Fusobacterium necrophorum, control cattle (n = 2) were inoculated with inactivated bacteria, and naturally affected cattle (n = 11) were found in a slaughterhouse.

Determination of α1 ag was made by use of a single radial immunodiffusion method. The action on lymphocyte blastogenesis was determined by [3H]thymidine incorporation. Cultured lymphocytes from healthy cattle were treated with variable concentrations of α1 ag purified from serum obtained from cattle with hepatic abscesses and suppression of blastogenesis stimulated by each of 3 mitogens was measured.

In cattle with experimentally induced abscesses, serum α1 ag concentration increased for 7 to 10 days after F necrophorum inoculation, its change being parallel to that of sialic acid. High concentration of α1 ag was found in naturally affected cattle and was highly correlated to sialic acid concentration. Suppression of lymphocyte blastogenesis in cattle with experimentally induced hepatic abscesses was highly correlated to serum α1 ag concentration.

Summary

Changes in serum alpha1-acid glycoprotein (α1 AG) concentration in cattle with hepatic abscesses were observed, and function of α1 ag was evaluated, particularly its influence on cellular immune response. Test cattle (n = 4) were inoculated with Fusobacterium necrophorum, control cattle (n = 2) were inoculated with inactivated bacteria, and naturally affected cattle (n = 11) were found in a slaughterhouse.

Determination of α1 ag was made by use of a single radial immunodiffusion method. The action on lymphocyte blastogenesis was determined by [3H]thymidine incorporation. Cultured lymphocytes from healthy cattle were treated with variable concentrations of α1 ag purified from serum obtained from cattle with hepatic abscesses and suppression of blastogenesis stimulated by each of 3 mitogens was measured.

In cattle with experimentally induced abscesses, serum α1 ag concentration increased for 7 to 10 days after F necrophorum inoculation, its change being parallel to that of sialic acid. High concentration of α1 ag was found in naturally affected cattle and was highly correlated to sialic acid concentration. Suppression of lymphocyte blastogenesis in cattle with experimentally induced hepatic abscesses was highly correlated to serum α1 ag concentration.

Advertisement