Characterization of a feline T-cell-specific monoclonal antibody reactive with a CD5-like molecule

Christopher D. Ackley From the Department of Pediatrics, Medicine and Microbiology, Division of Developmental and Clinical Immunology, The Comprehensive Cancer Center, School of Medicine, University of Alabama at Birmingham, 263 Wallace Tumor Institute, 1824 Sixth Ave S, UAB Station, Birmingham, AL, 35294-3300 (Ackley, Cooper), and the Howard Hughes Medical Institute, Birmingham, AL 35294 (Cooper). Dr. Cooper is an HHMI investigator.

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Max D. Cooper From the Department of Pediatrics, Medicine and Microbiology, Division of Developmental and Clinical Immunology, The Comprehensive Cancer Center, School of Medicine, University of Alabama at Birmingham, 263 Wallace Tumor Institute, 1824 Sixth Ave S, UAB Station, Birmingham, AL, 35294-3300 (Ackley, Cooper), and the Howard Hughes Medical Institute, Birmingham, AL 35294 (Cooper). Dr. Cooper is an HHMI investigator.

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Summary

The 43 monoclonal antibody raised against feline T cells was found to react with a single-chain glycoprotein of Mr 72,000 that is present on most thymocytes, 60% of lymph node cells, 20% of splenocytes, and 45% of blood mononuclear cells. All CD4+ and CD8+ T cells were found to express the 43-reactive determinant, as did a small subpopulation of CD4-/CD8-/AgM- lymphocytes in the periphery. The 43-reactive determinant was not detected on B cells, macrophages, or other types of blood cells. The 43 antigen was phosphorylated in resting and activated T cells. Its expression was upregulated by stimulation with phorbol myristate acetate and with phytohemagglutinin. When added to concanavalin A-stimulated T-cell cultures in low concentrations, the 43 antibody was found to augment mitogenesis. The data indicate that this antibody may identify a CD5 homologue on feline T cells.

Summary

The 43 monoclonal antibody raised against feline T cells was found to react with a single-chain glycoprotein of Mr 72,000 that is present on most thymocytes, 60% of lymph node cells, 20% of splenocytes, and 45% of blood mononuclear cells. All CD4+ and CD8+ T cells were found to express the 43-reactive determinant, as did a small subpopulation of CD4-/CD8-/AgM- lymphocytes in the periphery. The 43-reactive determinant was not detected on B cells, macrophages, or other types of blood cells. The 43 antigen was phosphorylated in resting and activated T cells. Its expression was upregulated by stimulation with phorbol myristate acetate and with phytohemagglutinin. When added to concanavalin A-stimulated T-cell cultures in low concentrations, the 43 antibody was found to augment mitogenesis. The data indicate that this antibody may identify a CD5 homologue on feline T cells.

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