Evaluation of total protein content in tears of dogs by polyacrylamide gel disk electrophoresis

Rafael Barrera From Departamento de Medicina y Sanidad Animal, Facultad de Veterinaria, Universidad de Extremadura, Carretera de Trujillo s/n, 10.071 Cáceres, Spain.

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Antonio Jiménez From Departamento de Medicina y Sanidad Animal, Facultad de Veterinaria, Universidad de Extremadura, Carretera de Trujillo s/n, 10.071 Cáceres, Spain.

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Rocío López From Departamento de Medicina y Sanidad Animal, Facultad de Veterinaria, Universidad de Extremadura, Carretera de Trujillo s/n, 10.071 Cáceres, Spain.

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María C. Ma ñé From Departamento de Medicina y Sanidad Animal, Facultad de Veterinaria, Universidad de Extremadura, Carretera de Trujillo s/n, 10.071 Cáceres, Spain.

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Jesús F. Rodríguez From Departamento de Medicina y Sanidad Animal, Facultad de Veterinaria, Universidad de Extremadura, Carretera de Trujillo s/n, 10.071 Cáceres, Spain.

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José M. Molleda From Departamento de Medicina y Sanidad Animal, Facultad de Veterinaria, Universidad de Extremadura, Carretera de Trujillo s/n, 10.071 Cáceres, Spain.

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Summary

Concentration of total proteins was measured and sodium dodecyl sulfate-polyacrylamide gel disk electrophoresis was performed on tear and plasma samples obtained from 26 healthy dogs, and the results were compared. Mean ± sem concentration of total proteins in tears was 0.63 ± 0.04 g/dl, and significant effects of age or gender were not found. The protein composition of tears in dogs was complex, and bands from light and heavy chains of immunoglobulins were identified by electrophoresis.

Summary

Concentration of total proteins was measured and sodium dodecyl sulfate-polyacrylamide gel disk electrophoresis was performed on tear and plasma samples obtained from 26 healthy dogs, and the results were compared. Mean ± sem concentration of total proteins in tears was 0.63 ± 0.04 g/dl, and significant effects of age or gender were not found. The protein composition of tears in dogs was complex, and bands from light and heavy chains of immunoglobulins were identified by electrophoresis.

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