Regulation of matrix metabolism in equine cartilage explant cultures by interleukin 1

Melinda H. MacDonald From the Departments of Veterinary Anatomy and Cell Biology (MacDonald, Stover) and Zoology (Benton) and the Division of Statistics (Willits), University of California, Davis, Davis, CA 95616.

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Susan M. Stover From the Departments of Veterinary Anatomy and Cell Biology (MacDonald, Stover) and Zoology (Benton) and the Division of Statistics (Willits), University of California, Davis, Davis, CA 95616.

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Neil H. Willits From the Departments of Veterinary Anatomy and Cell Biology (MacDonald, Stover) and Zoology (Benton) and the Division of Statistics (Willits), University of California, Davis, Davis, CA 95616.

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Hilary P. Benton From the Departments of Veterinary Anatomy and Cell Biology (MacDonald, Stover) and Zoology (Benton) and the Division of Statistics (Willits), University of California, Davis, Davis, CA 95616.

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Summary

Explant cultures were set up, using articular cartilage obtained from metatarsophalangeal joints of 11 horses. Explants from 2 horses were used to determine culture conditions appropriate for tissue viability. The cartilage explants maintained steady-state metabolism of proteoglycans during a 13-day evaluation period. The metabolic response of equine articular cartilage to incubation with recombinant human interleukin 1 (0.01 to 100 ng/ml) was studied, using cartilage obtained from the remaining 9 horses, age of which ranged from 3 months to 20 years. Interleukin 1 induced a dose-dependent release of glycosaminoglycan from the matrix during a 3-day incubation period. It also caused dose-dependent inhibition of glycosaminoglycan synthesis during a 3-hour pulse-labeling period. Expiants obtained from older horses were significantly (P < 0.05) less responsive to interleukin 1, with respect to synthesis and release of glycosaminoglycan.

Summary

Explant cultures were set up, using articular cartilage obtained from metatarsophalangeal joints of 11 horses. Explants from 2 horses were used to determine culture conditions appropriate for tissue viability. The cartilage explants maintained steady-state metabolism of proteoglycans during a 13-day evaluation period. The metabolic response of equine articular cartilage to incubation with recombinant human interleukin 1 (0.01 to 100 ng/ml) was studied, using cartilage obtained from the remaining 9 horses, age of which ranged from 3 months to 20 years. Interleukin 1 induced a dose-dependent release of glycosaminoglycan from the matrix during a 3-day incubation period. It also caused dose-dependent inhibition of glycosaminoglycan synthesis during a 3-hour pulse-labeling period. Expiants obtained from older horses were significantly (P < 0.05) less responsive to interleukin 1, with respect to synthesis and release of glycosaminoglycan.

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